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Muscular tubes of urethra engineered from adipose-derived stem cells and polyglycolic acid mesh in a bioreactor

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Abstract

We have explored the feasibility of using adipose-derived stem cells (ADSCs) and polyglycolic acid (PGA) for constructing muscular tubes of urethra in a bioreactor. With the induction of by 5-azacytidine, ADSCs were found to acquire a myoblast phenotype. Here we seeded ADSCs in a PGA mesh to construct the cell–PGA complex that was cultured statically for 1 week. Afterwards, the cell–PGA complex was subjected to extension stimulation in a bioreactor for 5 weeks. A muscular tube of urethra was formed after 6 weeks. Histological examination showed differentiated ADSCs and collagenous fibers had orientated well. This study demonstrates that tissue engineering of urethra tissues in vitro by using a bioreactor leads to tissue maturation and the differentiation of ADSCs. This novel technique could provide an effective approach for urethra tissue engineering.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (30973016) and Shanghai Natural Science Foundation (09ZR1424100).

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Authors and Affiliations

  1. Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Yi Shan Road 600, Shanghai, 200233, China

    Ying Wang, Qiang Fu & Ren-Yan Zhao

  2. Department of Plastic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, 200233, China

    Chen-Liang Deng

Authors
  1. Ying Wang
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  2. Qiang Fu
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  3. Ren-Yan Zhao
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  4. Chen-Liang Deng
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Correspondence to Qiang Fu.

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Wang, Y., Fu, Q., Zhao, RY. et al. Muscular tubes of urethra engineered from adipose-derived stem cells and polyglycolic acid mesh in a bioreactor. Biotechnol Lett 36, 1909–1916 (2014). https://doi.org/10.1007/s10529-014-1554-x

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  • DOI: https://doi.org/10.1007/s10529-014-1554-x

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