Abstract
Industrial wastewater from the production of sulfur containing esters and the resulting products of this synthesis, 2-ethylhexylthioglycolic acid (EHTG) and iso-octylthioglycolic acid (IOTG), were deployed in this study to enrich novel bacterial strains, since no wastewater and EHTG or IOTG degrading microorganisms were hitherto described or available. In addition, nothing is known about the biodegradation of these thiochemicals. The effect of this specific wastewater on the growth behaviour of microorganisms was investigated using three well-known Gram-negative bacteria (Escherichia coli, Pseudomonas putida, and Ralstonia eutropha). Concentrations of 5% (v/v) wastewater in complex media completely inhibited growth of these three bacterial strains. Six bacterial strains were successfully isolated, characterized and identified by sequencing their 16S rRNA genes. Two isolates referred to as Achromobacter sp. strain MT-E3 and Pseudomonas sp. strain MT-I1 used EHTG or IOTG, respectively, as well as the wastewater as sole source of carbon and energy for weak growth. More notably, both isolates removed these sulfur containing esters in remarkable amounts from the cultures supernatant. One further isolate was referred to as Klebsiella sp. strain 58 and exhibited an unusual high tolerance against the wastewater’s toxicity without utilizing the contaminative compounds. If cultivated with gluconic acid as additional carbon source, the strain grew even in presence of more than 40% (v/v) wastewater. Three other isolates belonging to the genera Bordetella and Pseudomonas tolerated these organic sulfur compounds but showed no degradation abilities.
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Abbreviations
- DSMZ:
-
Deutsche Sammlung von Mikroorganismen und Zellkulturen
- TG:
-
Thioglycolic acid
- EH:
-
2-Ethyl-1-hexanol
- MSM:
-
Mineral salt medium
- EHTG:
-
2-Ethylhexylthioglycolic acid
- IOTG:
-
Iso-octylthioglycolic acid
- LB:
-
Luria Bertani broth
- OSC:
-
Organic sulfur compounds
- Na:
-
Sodium
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Acknowledgments
The authors thank the company Bruno Bock Chemische Fabrik GmbH & Co KG (Niedermarschacht, Germany) for providing wastewater samples and information about its chemical composition as well as bulk containers of EHTG, IOTG and TG.
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Toups, M., Wübbeler, J.H. & Steinbüchel, A. Microbial utilization of the industrial wastewater pollutants 2-ethylhexylthioglycolic acid and iso-octylthioglycolic acid by aerobic Gram-negative bacteria. Biodegradation 21, 309–319 (2010). https://doi.org/10.1007/s10532-009-9302-y
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DOI: https://doi.org/10.1007/s10532-009-9302-y