Abstract
Coal gangue (CG), one of the world’s largest industrial solid wastes produced during coal mining, is extremely difficult to be used owing to its combined contents of clay minerals and organic macromolecules. This study explored a novel process of degrading the harmful organic compounds in the CG into humic acid using a biological method characterized by scanning electron microscope–energy dispersive spectrometer, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and elemental analyzer. The results reveal that adding selected Bacillus sp. to the CG for 40 days can increase the humic acid content by ~ 17 times, reaching 17338.17 mg/kg, which is also the best level for promoting plant growth. FTIR and XPS spectra show that the organic compounds in the CG transforms primarily from C=C to C=O, COOH, and O–H groups, indicating that the organic compounds are gradually oxidized and activated, improving the humic acid concentration of soil. In addition, Bacillus sp. decreases pH and benzo[a]pyrene contents, and increases the content of available nutrients. After microbial degradation, coal gangue can be turned into ecological restoration materials.
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The datasets generated during the current study are not publicly available but are available from the corresponding author on reasonable request.
References
Abdel-Shafy HI, Mansour MSM (2016) A review on polycyclic aromatic hydrocarbons: source, environmental impact, effect on human health and remediation. Egypt J Pet 25:107–123. https://doi.org/10.1016/j.ejpe.2015.03.011
Ahmad I, Ali S, Khan KS, Hassan FU, Ijaz SS, Bashir K (2015) Use of coal derived humic acid as soil conditioner for soil physical properties and its impact on wheat crop yield. Int J Biosci 6:81–89. https://doi.org/10.12692/ijb/6.12.81-89
Akimbekov N, Digel I, Qiao X, Tastambek K, Zhubanova A (2020) Lignite biosolubilization by Bacillus sp. RKB2 and characterization of its products. Geomicrobiol J 37(3):255–261. https://doi.org/10.1080/01490451.2019.1695022
Akimbekov N, Digel I, Abdieva G, Ualieva P, Tastambek K (2021) Lignite biosolubilization and bioconversion by Bacillus sp.: the collation of analytical data. Biofuels 12(3):247–258. https://doi.org/10.1080/17597269.2020.1753936
Ali J, Li Y, Wang X, Zhao J, Xi N, Zhang Z (2020) Climate-zone-dependent effect mechanism of humic acid and fulvic acid extracted from river sediments on aggregation behavior of graphene oxide. Sci Total Environ 721:137682. https://doi.org/10.1016/j.scitotenv.2020.137682
Aoyama M, Angers DA, N’Dayegamiye A, Bissonnette N (2000) Metabolism of 13C-labeled glucose in aggregates from soils with manure application. Soil Biol Biochem 32:295–300. https://doi.org/10.1016/S0038-0717(99)00152-2
Authority EFS (2008) Polycyclic aromatic hydrocarbons in food-scientific opinion of the panel on contaminants in the food chain. EFSA J 6:724. https://doi.org/10.2903/j.efsa.2008.724
Baird WM, Hooven LA, Mahadevan B (2005) Carcinogenic polycyclic aromatic hydrocarbon-DNA adducts and mechanism of action. Environ Mol Mutagen 45:106–114. https://doi.org/10.1002/em.20095
Basaran Y, Denizli A, Sakintuna B, Taralp A, Yürüm Y (2003) Bio-liquefaction/solubilization of low-rank turkish lignites and characterization of the products. Energy Fuels 17:1068–1074. https://doi.org/10.1021/ef020210s
Bonilla M, Olivaro C, Corona M, Vazquez A, Soubes M (2005) Production and characterization of a new bioemulsifier from Pseudomonas putida ML2. J Appl Microbiol 98(2):456–463. https://doi.org/10.1111/j.1365-2672.2004.02480.x
de Melo BAG, Motta FL, Santana MHA (2016) Humic acids: structural properties and multiple functionalities for novel technological developments. Mater Sci Eng C 62:967–974. https://doi.org/10.1016/j.msec.2015.12.001
Dong LH, Yuan Q, Yuan HL (2006) Changes of chemical properties of humic acids from crude and fungal transformed lignite. Fuel 85(17–18):2402–2407. https://doi.org/10.1016/j.fuel.2006.05.027
Dong LH, Yang JS, Yuan HL, Wang ET, Chen WX (2008) Chemical characteristics and influences of two fractions of chinese lignite humic acids on urease. Eur J Soil Biol 44:166–171. https://doi.org/10.1016/j.ejsobi.2007.07.002
Frederico GAD, Amanda G, Antônio CP, Gomes Marysilvia FC, Maria LMR (2022) Using XPS and FTIR spectroscopies to investigate polyamide 11 degradation on aging flexible risers. Polym Degrad Stab 195:109787. https://doi.org/10.1016/j.polymdegradstab.2021.109787
Gao YJ, Huang HJ, Tang WJ, Liu XY, Yang XY, Zhang JB (2015) Preparation and characterization of a novel porous silicate material from coal gangue. Microporous Mesoporous Mater 217:210–218. https://doi.org/10.1016/j.micromeso.2015.06.033
Ghani MJ, Rajoka MI, Akhtar K (2015) Investigations in fungal solubilization of coal: mechanisms and significance. Biotechnol Bioprocess Eng 20(4):634–642. https://doi.org/10.1007/s12257-015-0162-5
Gholami H, Raouf FF, Saharkhiz MJ, Ghani A (2018) Yield and physicochemical properties of inulin obtained from iranian chicory roots under vermicompost and humic acid treatments. Ind Crop Prod 123:610–616. https://doi.org/10.1016/j.indcrop.2018.07.031
Hayatsu R, Winans RE, Scott RG, Moore LP, Studier MH (1978) Characterization of organic acids trapped in coals. Nature 275:116–118. https://doi.org/10.1038/275116a0
Herbstman JB (2012) Prenatal exposure to polycyclic aromatic hydrocarbons, benzo[a]pyrene–DNA adducts, and genomic DNA methylation in cord blood. Environ Health Perspect 120:733–738. https://doi.org/10.1289/ehp.1104056
Hölker U, Schmiers H, Große S, Winkelhöfer M, Polsakiewicz M, Ludwig S (2003) Solubilization of low-rank coal by Trichoderma atroviride: evidence for the involvement of hydrolytic and oxidative enzymes by using 14C-labelled lignite. J Ind Microbiol Biotechnol 28(4):207–212. https://doi.org/10.1016/s0140-6701(03)90552-2
Hua CY, Zhou GZ, Yin X, Wang CZ, Chi BR, Cao YY, Wang Y, Zheng Y, Cheng ZR, Li RY (2018) Assessment of heavy metal in coal gangue, distribution, leaching characteristic and potential ecological risk. Environ Sci Pollut Res 25(32):32321–32331. https://doi.org/10.1007/s11356-018-3118-4
Jiang F, Li ZH, Lv ZW, Gao TG, Yang JS, Qin ZH, Yuan LH (2013) The biosolubilization of lignite by Bacillus sp. Y7 and characterization of the soluble products. Fuel 103:639–645. https://doi.org/10.1016/j.fuel.2012.08.030
Klein J (1999) Biological processing of fossil fuels. Appl Microbiol Biotechnol 52(1):2–15. https://doi.org/10.1016/S0378-3820(97)80218-4
Li JY, Wang JM (2019) Comprehensive utilization and environmental risks of coal gangue: a review. J Clean Prod 239:117946. https://doi.org/10.1016/j.jclepro.2019.117946
Li JC, Zeng XQ, Ren TH, Van der Heide E (2014) The preparation of graphene oxide and its derivatives and their application in bio-tribological systems. Lubricants 2:137–161. https://doi.org/10.3390/lubricants2030137
Liang LC, Xu XH, Han JL, Xu ZD, Wu P, Guo JY, Qiu GL (2019) Characteristics, speciation, and bioavailability of mercury and methylmercury impacted by an abandoned coal gangue in southwestern China. Environ Sci Pollut Res 26(36):37001–37011. https://doi.org/10.1007/s11356-019-06775-7
Lipczynska-Kochany E (2018) Humic substances, their microbial interactions and effects on biological transformations of organic pollutants in water and soil: a review. Chemosphere 202:420–437. https://doi.org/10.1016/j.chemosphere.2018.03.104
Liu M, Wang C, Wang F, Xie Y (2019) Maize (Zea mays) growth and nutrient uptake following integrated improvement of vermicompost and humic acid fertilizer on coastal saline soil. Appl Soil Ecol 142:147–154. https://doi.org/10.1016/j.apsoil.2019.04.024
Maccarthy P (2001) The principles of humic substances: an introduction to the first principle. Soil Sci 166:738–751. https://doi.org/10.1097/00010694-200111000-00003
Machnikowska H, Pawelec K, Podgorska A (2002) Microbial degradation of low rank coals. Fuel Process Technol 77:17–23. https://doi.org/10.1016/S0378-3820(02)00064-4
Najafi AR, Rahimpour MR, Roostaazad R, Arabian D, Ghobadi Z (2002) Enhancing biosurfactant production from an indigenous strain of Bacillus mycoides by optimizing the growth conditions using a response surface methodology. Chem Eng J 163:188–194. https://doi.org/10.1016/j.cej.2010.06.044
Ni Y, Wang P, Song H, Lin X, Kokot S (2014) Electrochemical detection of benzo(a)pyrene and related DNA damage using DNA/hemin/nafion–graphene biosensor. Anal Chim Acta 821:34–40. https://doi.org/10.1016/j.aca.2014.03.006
Oboirien BO, Burton SG, Cowan D, Harrison STL (2008) The effect of the particulate phase on coal biosolubilisation mediated by Trichoderma atroviride in a slurry bioreactor. Fuel Process Technol 89(2):123–130. https://doi.org/10.1016/j.fuproc.2007.06.019
Pang AY, Song FP, Song XL, Guo XS, Lu YY, Chen SG, Zhu FJ, Zhang ND, Zou JC, Zhang PH (2021) Effects of different types of humic acid isolated from coal on soil NH3 volatilization and CO2 emissions. Environ Res 194:110711. https://doi.org/10.1016/j.envres.2021.110711
Romanowska I, Strzelecki B, Bielecki S (2015) Biosolubilization of Polish brown coal by Gordonia alkanivorans S7 and Bacillus mycoides NS1020. Fuel Process Technol 131:430–436. https://doi.org/10.1016/j.fuproc.2014.12.019
Sabar MA, Ali MI, Fatima N, Malik AY, Jamal A, Liaquat R, He H, Liu FJ, Guo HG, Urynowicz M, Huang ZX (2020) Evaluation of humic acids produced from pakistani subbituminous coal by chemical and fungal treatments. Fuel 278:118301. https://doi.org/10.1016/j.fuel.2020.118301
Selvi AV, Banerjee R, Ram LC, Singh G (2009) Biodepolymerization studies of low rank indian coals. World J Microbiol Biotechnol 25:1713–1720. https://doi.org/10.1007/s11274-009-0066-7
Silva-Stenico ME, Vengadajellum CJ, Janjua HA, Harrison STL, Burton SG, Cowan DA (2007) Degradation of low rank coal by Trichoderma atroviride ES11. J Ind Microbiol Biotechnol 34(9):625–631. https://doi.org/10.1007/s10295-007-0242-4
Stevenson FJ (1995) Humus chemistry: genesis, composition, reactions, second edition. J Chem Educ 72:A93. https://doi.org/10.1021/ed072pA93.6
Stout SA (1992) Aliphatic and aromatic triterpenoid hydrocarbons in a tertiary angiospermous lignite. Org Geochem 18(1):51–66. https://doi.org/10.1016/0146-6380(92)90143-L
Turgay O, Naml A, Unver S, Tamer N (2011) Effects of coal derived humic substance on some soil properties and bread wheat yield. Commun Soil Sci Plant Anal 42:1050–1070. https://doi.org/10.1080/00103624.2011.562586
Wenzl T, Simon R, Anklam E, Kleiner J (2006) Analytical methods for polycyclic aromatic hydrocarbons (PAHs) in food and the environment needed for new food legislation in the European Union. Trends Anal Chem 25:716–725. https://doi.org/10.1016/j.trac.2006.05.010
Yang M, Guo ZX, Deng YS, Xing XL, Qiu KH, Long JP, Li JF (2012) Preparation of CaO–Al2O3–SiO2 glass ceramics from coal gangue. Int J Miner Process 102(5):112–115. https://doi.org/10.1016/j.minpro.2011.11.004
Yi C, Ma HQ, Chen HY, Wang JX, Shi J, Li ZH, Yu MK (2018) Preparation and characterization of coal gangue geopolymers. Constr Build Mater 187:318–326. https://doi.org/10.1016/j.conbuildmat.2018.07.220
Young CC, Su CH, Li GC, Wang MC, Arun AB (2004) Prospects for nitrogen incorporation into humic acid as evidenced by alkaline extraction method. Curr Sci. https://doi.org/10.1073/pnas.0409058101
Yu L, Wang PT, Xu QT, He T, Oduro G, Lu Y (2019) Enhanced decolorization of methyl orange by Bacillus sp. strain with magnetic humic acid nanoparticles under high salt conditions. Bioresour Technol 288:121535. https://doi.org/10.1016/j.biortech.2019.121535
Yuan H, Yang J, Chen W (2006) Production of alkaline materials, surfactants and enzymes by Penicillium decumbens strain P6 in association with lignite degradation/solubilization. Fuel 85:1378–1382. https://doi.org/10.1016/j.fuel.2005.12.003
Zhan S, Zhang X, Cao S, Huang J (2015) Benzo(a)pyrene disrupts mouse preimplantation embryo development. Fertil Steril 103:815–825. https://doi.org/10.1016/j.fertnstert.2014.11.013
Acknowledgements
This work was supported by Ningxia Hui Autonomous Region’s Key Research and Development Plan (No.2021BEE02019), the Ningxia Hui Autonomous Region’s Key tasks of industrial innovation (No.2021010401), and Beijing Natural Science Foundation (No.2222079), the Foundation of Innovation Academy for Green Manufacture Institute, Chinese Academy of Sciences (IAGM2022D06).
Funding
This work was supported by Ningxia Hui Autonomous Region’s Key Research and Development Plan (No.2021BEE02019), the Ningxia Hui Autonomous Region’s Key tasks of industrial innovation (No.2021010401), and Beijing Natural Science Foundation (No.2222079), the Foundation of Innovation Academy for Green Manufacture Institute, Chinese Academy of Sciences (IAGM2022D06).
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Liu, C., Ma, S., Wang, X. et al. Biodegradation of organic compounds in the coal gangue by Bacillus sp. into humic acid. Biodegradation 34, 125–138 (2023). https://doi.org/10.1007/s10532-022-10007-0
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DOI: https://doi.org/10.1007/s10532-022-10007-0