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Investigation of osteogenic activity of primary rabbit periosteal cells stimulated by multi-axial tensile strain

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Abstract

Periosteum–derived cells was indicated to respond to mechanical force and have stem cell potential capable of differentiating into multiple tissue. Investigation of osteogenic activity under mechanical stimulation is important to understand the therapeutic conditions of fracture healing. In this work, a cell culture platform was developed for respectively providing isotropic and anisotropic axial strain. Primary rabbit periosteal cells were isolated and cultured in the chamber. Multi-axial tensile strain was received and osteogenic activity was investigated by mRNA expressions of CBFA1 and OPN. The highest mRNA expression was found in moderate strain (5-8%) under anisotropic axial strain. These results provided important foundation for further in vivo studies and development of tailor-made stretching rehabilitation equipment.

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Acknowledgements

This work was supported by Chang Gung Memorial Hospital, Linkou branch, Taiwan (Project no. BMRPC05).

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Authors and Affiliations

  1. Bone and Joint Research Center, Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Tooyuan Branch, Taoyuan, Taiwan

    Chih-Hao Chiu & Alvin Chao-Yu Chen

  2. Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan

    Jun-Liang Liu, Chih-Hsuan Chang & Kin Fong Lei

  3. Department of Mechanical Engineering, Chang Gung University, Taoyuan, Taiwan

    Kin Fong Lei

  4. Department of Radiation Oncology, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan, Taiwan

    Kin Fong Lei

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  1. Chih-Hao Chiu
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Correspondence to Kin Fong Lei or Alvin Chao-Yu Chen.

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Chiu, CH., Liu, JL., Chang, CH. et al. Investigation of osteogenic activity of primary rabbit periosteal cells stimulated by multi-axial tensile strain. Biomed Microdevices 19, 13 (2017). https://doi.org/10.1007/s10544-017-0154-7

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  • DOI: https://doi.org/10.1007/s10544-017-0154-7

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