Abstract
Mesenchymal stem cells, being characterized by high self-renewal capacity and multi-lineage differentiation potential, are widely used in regenerative medicine especially for repair of bone defects in patients with poor bone regenerative capacity. In this study, we aimed to compare the osteogenic potential of human maxillary schneiderian sinus membrane (hMSSM)-derived stem cells versus permanent teeth dental pulp stem cells (DPSCs). Both cells types were cultivated in osteogenic and non-osteogenic inductive media. Alkaline phosphatase (ALP) activity assay and quantitative real-time PCR analysis were carried out to assess osteogenic differentiation. We showed that ALP activity and osteoblastic markers transcription levels were more striking in hMSSM-derived stem cells than DPSCs. Our results highlight hMSSM-derived stem cells as a recommended stem cell type for usage during bone tissue regenerative therapy.
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This work was supported by grants from Lebanese University (18840 and 5740-4).
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This study was approved by the Institutional Review Board of the Lebanese University (CUEMB 1/2014- and 64-4-2016 and 62/2016). The sinus maxillary membrane protocol is registered in the Clinical Trial.gov (ID NCT02676921). All experiments were conducted in compliance with current Good Clinical Practice standards and in accordance with relevant guidelines and regulations and the principles set forth under the Declaration of Helsinki (1989).
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Antoine Berbéri and Joseph Sabbagh are co-first authors.
Mohammad Fayyad-kazan and Bassam Badran are joint senior co-authors.
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Berbéri, A., Sabbagh, J., Bou Assaf, R. et al. Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study. Cell Tissue Bank 22, 409–417 (2021). https://doi.org/10.1007/s10561-020-09887-4
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DOI: https://doi.org/10.1007/s10561-020-09887-4