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Adrenomedullin Improves the Blood–Brain Barrier Function Through the Expression of Claudin-5

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Summary

1. Aims: Brain vascular endothelial cells secret Adrenomedullin (AM) has multifunctional biological properties. AM affects cerebral blood flow and blood–brain barrier (BBB) function. We studied the role of AM on the permeability and tight junction proteins of brain microvascular endothelial cells (BMEC).

2. Methods: BMEC were isolated from rats and a BBB in vitro model was generated. The barrier functions were studied by measuring the transendothelial electrical resistance (TEER) and the permeability of sodium fluorescein and Evans’ blue albumin. The expressions of tight junction proteins were analyzed using immunocytochemistry and immunoblotting.

3. Results: AM increased TEER of BMEC monolayer dose-dependently. Immunocytochemistry revealed that AM enhanced the claudin-5 expression at a cell–cell contact site in a dose-dependent manner. Immunoblotting also showed an overexpression of claudin-5 in AM exposure.

4.Conclusions: AM therefore inhibits the paracellular transport in a BBB in vitro model through claudin-5 overexpression.

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Authors and Affiliations

  1. Department of Neurosurgery, Nagasaki University School of Medicine, Nagasaki, Japan

    Masaru Honda, Kentaro Hayashi, Naoki Kitagawa, Keisuke Tsutsumi & Izumi Nagata

  2. Department of Pharmacology 1, Nagasaki University School of Medicine, Nagasaki, Japan

    Shinsuke Nakagawa & Masami Niwa

  3. 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan

    Masaru Honda

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  1. Masaru Honda
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  2. Shinsuke Nakagawa
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  3. Kentaro Hayashi
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  4. Naoki Kitagawa
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  7. Masami Niwa
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Correspondence to Masaru Honda.

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Honda, M., Nakagawa, S., Hayashi, K. et al. Adrenomedullin Improves the Blood–Brain Barrier Function Through the Expression of Claudin-5. Cell Mol Neurobiol 26, 109–118 (2006). https://doi.org/10.1007/s10571-006-9028-x

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  • DOI: https://doi.org/10.1007/s10571-006-9028-x

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