Abstract
The generation of free radicals and oxidative stress has been linked to several neurodegenerative diseases including Parkinson’s disease, Alzheimer’s disease, Huntington’s disease, and Amyotrophic lateral sclerosis. The use of free radical scavenging molecules for the reduction of intracellular reactive oxygen species is one of the strategies used in the clinical management of neurodegeneration. Fungal secondary metabolism is a rich source of novel molecules with potential bioactivity. In the current study, bikaverin was extracted from Fusarium oxysporum f. sp. lycopersici and its structural characterization was carried out. Further, we explored the protective effects of bikaverin on oxidative stress and its anti-apoptotic mechanism to attenuate H2O2-induced neurotoxicity using human neuroblastoma SH-SY5Y cells. Our results elucidate that pretreatment of neurons with bikaverin attenuates the mitochondrial and plasma membrane damage induced by 100 µM H2O2 to 82 and 26 % as evidenced by MTT and LDH assays. H2O2 induced depletion of antioxidant enzyme status was also replenished by bikaverin which was confirmed by Realtime Quantitative PCR analysis of SOD and CAT genes. Bikaverin pretreatment efficiently potentiated the H2O2-induced neuronal markers, such as BDNF, TH, and AADC expression, which orchestrate the neuronal damage of the cell. The H2O2-induced damage to cells, nuclear, and mitochondrial integrity was also restored by bikaverin. Bikaverin could be developed as a preventive agent against neurodegeneration and as an alternative to some of the toxic synthetic antioxidants.
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Andersen JK (2004) Oxidative stress in neurodegeneration: cause or consequence? Nat Med 5:S18–S25
Balan J, Fuska J, Kuhr I, Kuhrová V (1970) Bikaverin, an antibiotic from Gibberella fujikuroi, effective against Leishmania brasiliensis. Folia Microbiol 15:479–484
Baraccaa A, Gianluca S, Giancarlo S, Giorgio L (2003) Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F0 during ATP synthesis. Biochim Biophys Acta 1606:137–146
Bell AA, Wheeler MH, Liu J, Stipanovic RD, Puckhaber LS, Orta H (2003) United States Department of Agriculture-Agricultural Research Service. Studies on polyketide toxins of Fusarium oxysporum f. sp. vasinfectum: potential targets for disease control. Pest Manag Sci 59:736–747
Brewer D, Arsenault GP, Wright C, Vining LC (1973) Production of bikaverin by Fusarium oxysporum and its identity with lycopersin. J Antibiot 26:778–781
Carmen LM, Roberto RO, Javier A (2010) Bikaverin production and applications. Appl Microbiol Biotechnol 87:21–29
Casson RJ, Chidlow G, Ebneter A, Wood JP, Crowston J, Goldberg I (2012) Translational neuroprotection research in glaucoma: a review of definitions and principles. Clin Exp Ophthalmol 40(4):350–357
Chen WG, Chang Q, Lin Y, Meissner A, West AE, Griffith EC, Jaenisch R, Greenberg ME (2003) Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2. Science 302:885–889
Cho HS, Kim S, Lee SY, Park JA, Kim SJ, Chun HS (2008) Protective effect of the green tea component, l-theanine on environmental toxins-induced neuronal cell death. Neurotoxicology 29(4):656–662
Cho ES, Jang YJ, Hwang MK, Kang NJ, Lee KW, Lee HJ (2009) Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals. Mutat Res 661:18–24
Choi BS, Sapkota K, Kim S, Lee HJ, Choi HS, Kim SJ (2010) Antioxidant activity and protective effects of Tripterygium regelii extract on hydrogen peroxide-induced injury in human dopaminergic cells, SH-SY5Y. Neurochem Res 35:1269–1280
Denisova NA, Cantuti-Castelvetri I, Haasan WN, Paulson KE, Joseph JA (2001) Role of membrane lipids in regulation of vulnerability to oxidative stress in PC12 cells: implication for aging. Free Radic Biol Med 30:671–678
Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S (1998) A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391:43–50
Fernandez SE, Canizares FJ, Cubero MA, Warley A, Campos A (1999) Changes in elemental content during apoptotic cell death studied by electron probe X-ray microanalysis. Exp Cell Res 253:454–462
Forman HJ (2007) Use and abuse of exogenous H2O2 in studies of signal transduction. Free Radic Biol Med 42:926–932
Freidovich I (1999) Fundamental aspects of reactive oxygen species, or what’s the matter with oxygen? Ann N Y Acad Sci 893:13
Friedman J (2011) Why is the nervous system vulnerable to oxidative stress? In: Gadoth N, Gobel HH (eds) Oxidative stress in applied basic research and clinical practice. The Humana Press, New York, pp 19–27
Ghaffari H, Ghassam BJ, Chandra Nayaka S, Ramachandra Kini K, Prakash HS (2014) Antioxidant and neuroprotective activities of Hyptis suaveolens (L.) Poit. against oxidative stress-induced neurotoxicity. Cell Mol Neurobiol. doi:10.1007/s10571-013-0016-7
Gilgun-Sherki Y, Melamed E, Offen D (2001) Oxidative stress induced-neurodegenerative diseases: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacology 40:959–975
Gutteridge JMC (1995) Lipid peroxidation and antioxidants as biomarker of tissue damage. Clin Chem 41:1819–1828
Halliwell B, Aruoma OI (1991) DNA damage by oxygen derived species. Its mechanism and measurement in mammalian systems. FEBS Lett 281:9–19
Hemanth Kumar K, Khanum F (2013) Hydroalcoholic extract of Cyperus rotundus ameliorates H2O2-induced human neuronal cell damage via its anti-oxidative and anti-apoptotic machinery. Cell Mol Neurobiol 33:5–17
Hiort J, Maksimenka K, Reichert M, Perovic-Ottstadt S, Lin WH, Wray V, Steube K, Schaumann K, Weber H, Proksch P, Ebel R, Müller WEG, Bringmann G (2004) New natural products from the sponge-derived fungus Aspergillus niger. J Nat Prod 67:1532–1543
Hyejin L, Jinhee K, Seo YL, Jeong HP, Gwi SH (2012) Processed Panax ginseng, Sun Ginseng, decreases oxidative damage induced by tert-butyl hydroperoxide via regulation of antioxidant enzyme and anti-apoptotic molecules in HepG2 cells. J Ginseng Res 36(3):248–255
Joshi DC, Bakowska JC (2011) Determination of mitochondrial membrane potential and reactive oxygen species in live rat cortical neurons. J Vis Exp 51:e2704
Kassie F, Parzefall W, Knasmuller S (2000) Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res 463:13–31
Kim SH, Ko H, Bang HS, Park SH, Kim DG, Kwon HC, Kim SY, Shin J, Oh DC (2011) Coprismycins A and B, neuroprotective phenylpyridines from the dung beetle-associated bacterium, Streptomyces sp. Bioorg Med Chem Lett 21:5715–5718
Klein JA, Susan LA (2003) Oxidative stress, cell cycle, and neurodegeneration. J Clin Invest 111:785–793
Kovac L, Bohmerova E, Fuska J (1978) Inhibition of mitochondrial functions by the antibiotics bikaverin and duclauxine. J Antibiot 31:616–620
Kovacsova M, Barta A, Parohova J, Vrankova S, Pechanova O (2010) Neuroprotective mechanisms of natural polyphenolic compounds. Act Nerv Super Rediviva 52(3):181–186
Kumar KH, Tamatam A, Pal A, Khanum F (2013) Neuroprotective effects of Cyperus rotundus on SIN-1 induced nitric oxide generation and protein nitration: ameliorative effect against apoptosis mediated neuronal cell damage. Neurotoxicology 34:150–159
Kwon HR, Son SW, Han HR, Choi GJ, Jang KS, Choi YH, Lee S, Sung ND, Kim JC (2007) Nematicidal activity of bikaverin and fusaric acid isolated from Fusarium oxysporum against pine wood nematode, Bursaphelenchus xylophilus. Plant Pathol J 23:318–321
Kwon SH, Kim MJ, Ma SX, You IJ, Hwang JY, Oh JH, Kim SY, Kim HC, Lee SY, Jang CG (2012) Eucommia ulmoides Oliv. Bark. protects against hydrogen peroxide-induced neuronal cell death in SH-SY5Y cells. J Ethnopharmacol 142(2):337–345
Lau FC, Shukitt-Hale B, Joseph JA (2005) The beneficial effects of fruit polyphenols on brain aging. Neurobiol Aging 26:128–132
Lee CH, Hwang DS, Kim HG, Oh H, Park H, Cho JH, Lee JM, Jang JB, Lee KS, Oh MS (2010) Protective effect of Cyperi rhizoma against 6-hydroxydopamine-induced neuronal damage. J Med Food 13:564–571
Li J, Li W, Su J, Liu W, Altura BT, Altura BM (2003) Hydrogen peroxide induces apoptosis in cerebral vascular smooth muscle cells: possible relation to neurodegenerative diseases and strokes. Brain Res Bull 62:101–106
Lin Z, Wen J, Zhu T, Fang Y, Gu Q, Zhu W (2008) Chrysogenamide A from an endophytic fungus associated with Cistanche deserticola and its neuroprotective effect on SH-SY5Y Cells. J Antibiot 61(2):81–85
MacNee W, Rahman I (2001) Is oxidative stress central to the pathogenisis of chronic obstructive pulmonary disease? Trends Mol Med 7:55–62
Marchesi E, Rota C, Fann YC, Chignell CF, Mason RP (1999) Photoreduction of the fluorescent dye 2′,7′-dichlorofluorescein: a spin trapping and direct electron spin resonance study with implications for oxidative stress measurements. Free Radic Biol Med 26:148–161
Mario EG, Gabriella K, Peter R, Moussa BHY (1994) Oxidative stress: free radical production in neural degeneration. Pharmacol Ther 63(1):37–122
Martin S, Gonzalez-Burgos E, Carretero ME, Gomez-Serranillos MP (2011) Neuroprotective properties of Spanish red wine and its isolated polyphenols on astrocytes. Food Chem 128:40–48
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
Muller DPR (1996) Neurological disease. Adv Pharmacol 38:557–580
Park SE, Kim S, Kumar S, Kim SJ (2010) Neuroprotective effect of Rosmarinus officinalis extract on human dopaminergic cell line, SH-SY5Y. Cell Mol Neurobiol 30:759–767
Pechanova O, Bernatova I, Babal P, Martinez MC, Kysela S, Stvrtina S, Andriantsitohaina R (2004) Red wine polyphenols prevent cardiovascular alterations in L-NAME-induced hypertension. J Hypertens 22:155–159
Rastogi RP, Shailendra PS, Donat PH, Rajeshwar PS (2010) Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochem Biophys Res Commun 397:603–607
Ritz MF, Ratajczak P, Curin Y, Cam E, Mendelowitsch A, Pinet F, Andriantsitohaina R (2008) Chronic treatment with red wine polyphenol compounds mediates neuroprotection in a rat model of ischemic cerebral stroke. J Nutr 138(3): 519–525
Sapkota K, Kim S, Park SE, Kim SJ (2011) Detoxified extract of Rhus verniciflua stokes inhibits rotenone-induced apoptosis in human dopaminergic cells, SH-SY5Y. Cell Mol Neurobiol 31(2):213–223
Schapira AH (1999) Science, medicine, and the future: Parkinson’s disease. Br Med J 318:311–314
Seidl SE, Potashkin JA (2011) The promise of neuroprotective agents in Parkinson’s disease. Front Neurol 2(68):1–19
Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191
Son SW, Kim HY, Choi GJ, Lim HK, Jang KS, Lee SO, Lee S, Sung ND, Kim JC (2008) Bikaverin and fusaric acid from Fusarium oxysporum show antioomycete activity against Phytophthora infestans. J Appl Microbiol 104:692–698
Spencer JP (2010) The impact of fruit flavonoids on memory and cognition. Br J Nutr 104(3):S40–S47
Sunil AG, Kesavanarayanan KS, Kalaivani P, Sathiya S, Ranju V, Jyothi Priya R, Pramila B, Solomon Paul FD, Venkhatesh J, Saravana Babu C (2011) Total oligomeric flavonoids of Cyperus rotundus ameliorates neurological deficits, excitotoxicity and behavioral alterations induced by cerebral ischemic-reperfusion injury in rats. Brain Res Bull 84:394–405
Susanne BS, Andreas H, Stefan P, Gunter S (2005) The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 20(4):245–254
Thomas RB, Joy S, Ajayan MS, Paulose CS (2013) Neuroprotective potential of Bacopa monnieri and Bacoside A Against dopamine receptor dysfunction in the cerebral cortex of neonatal hypoglycaemic rats. Cell Mol Neurobiol 33(8):1065–1074
Uttara B, Singh AV, Zamboni P, Mahajan RT (2009) Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 7:65–74
Valko M, Dieter L, Jan M, Mark TDC, Milan M, Joshua T (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39(1):44–84
van Acker FA, Schouten O, Haenen GR, van der Vijgh WJ, Bast A (2000) Flavonoids can replace alpha-tocopherol as an antioxidant. FEBS Lett 473:145–148
Venkataramana M, Chandranayaka S, Anand T, Ayaz M, Rajesh R, Divakara ST, Murali HS, Praksh HS, Rao PVL, Balakrishna K, Murali HS, Batra HV (2014) Zearalenone induced toxicity in SH-SY5Y cells: the role of oxidative stress evidenced by N-acetyl cysteine. Food Chem Toxicol 65:335–342
Wang Q, Yan J, Chen X, Li J, Yang Y, Weng J, Deng C, Yenari MA (2011) Statins: multiple neuroprotective mechanisms in neurodegenerative diseases. Exp Neurol 230(1):27–34
Zádori D, Klivényi P, Szalárdy L, Fülöp F, Toldi J, Vécsei L (2012) Mitochondrial disturbances, excitotoxicity, neuroinflammation and kynurenines: novel therapeutic strategies for neurodegenerative disorders. J Neurol Sci 322(1):187–191
Zhan J, Burns AM, Liu MX, Faeth SH, Gunatilaka AA (2007) Search for cell motility and angiogenesis inhibitors with potential anticancer activity: beauvericin and other constituents of two endophytic strains of Fusarium oxysporum. J Nat Prod 70:227–232
Zhang F, Shi JS, Zhou H, Wilson B, Hong JS, Gao HM (2010) Resveratrol protects dopamine neurons against lipopolysaccharide induced neurotoxicity through its anti-inflammatory actions. Mol Pharmacol 78(3):466–477
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The authors are grateful to UGC (University Grants Commission), New Delhi, India for providing the financial support under UGC-Major Research Project F. No. 34-244/2008 (SR). Authors are thankful to the Director DFRL, for providing necessary facilities for conducting the experiments.
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D. Nirmaladevi and M. Venkataramana have contributed equally to this study.
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Nirmaladevi, D., Venkataramana, M., Chandranayaka, S. et al. Neuroprotective Effects of Bikaverin on H2O2-Induced Oxidative Stress Mediated Neuronal Damage in SH-SY5Y Cell Line. Cell Mol Neurobiol 34, 973–985 (2014). https://doi.org/10.1007/s10571-014-0073-6
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DOI: https://doi.org/10.1007/s10571-014-0073-6