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A plea to reduce or replace fetal bovine serum in cell culture media

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References

  • Anderson NL, Anderson NG (2002) The human plasma proteome. History, character, and diagnostic prospects. Mol Cell Proteomics 1:845–867

    Article  CAS  Google Scholar 

  • Anderson NL, Polanski M, Pieper R, Gatlin T, Tirumalai RS, Conrads TP, Veenstra TD, Adkins JN, Pounds JG, Fagan R, Lobley A (2004) The human plasma proteome: a nonredundant list developed by combination of four separate sources. Mol Cell Proteomics 3:311–326

    Article  CAS  Google Scholar 

  • Asher DM (1999) Bovine sera used in the manufacture of biologicals: current concerns and policies of the US Food and Drug Administration regarding the transmissible spongiform encephalopathies. Dev Biol Stand 99:41–44

    CAS  Google Scholar 

  • Bohn B (1995) Fatal bovine serum. Nat Biotechnol 13:926–927

    Article  CAS  Google Scholar 

  • Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G (2010) Serum-free cell culture: the serum-free media interactive online database. ALTEX 27:53–62

    Google Scholar 

  • Dormont D (1999) Transmissible spongiform encephalopathy agents and animal sera. Dev Biol Stand 99:25–34

    CAS  Google Scholar 

  • Even MS, Sandusky CB, Barnard ND (2006) Serum-free hybridoma culture: ethical, scientific and safety considerations. Trends Biotechnol 24:105–108

    Article  CAS  Google Scholar 

  • FDA (2013) US Food and Drug Administration; http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfRes/res.cfm?ID=117863

  • Fujimoto B (2002) Fetal bovine serum—supply vs. demand? Art Sci 21:1–4

    Google Scholar 

  • Gstraunthaler G (2003) Alternatives to the use of fetal bovine serum: serum-free cell culture. ALTEX 20:275–281

    Google Scholar 

  • Gstraunthaler G, Lindl T (2013) Zell- und Gewebekultur, 7th edn. Spektrum Springer Verlag, Heidelberg

    Book  Google Scholar 

  • Hodgson J (1991) Checking the sources: the serum supply secret. Nat Biotechnol 9:1320–1324

    Article  CAS  Google Scholar 

  • Hodgson J (1993) Fetal bovine serum revisited. Nat Biotechnol 11:49–53

    Article  CAS  Google Scholar 

  • Hodgson J (1995) To treat or not to treat: that is the question for serum. Nat Biotechnol 13:333–343

    Article  CAS  Google Scholar 

  • Jochems CE, van der Valk JBF, Stafleu FR, Baumans V (2002) The use of fetal bovine serum: ethical and scientific problem? ATLA 30:219–227

    CAS  Google Scholar 

  • Nielsen O (1995) Changing serum’s mind-set. Nat Biotechnol 13:626

    Article  CAS  Google Scholar 

  • Psychogios N, Hau DD, Peng J, Guo AC, Mandal R, Bouatra S, Sinelnikov I, Krishnamurthy R, Eisner R, Gautam B, Young N, Xia J, Knox C, Dong E, Huang P, Hollander Z, Pedersen TL, Smith SR, Bamforth F, Greiner R, McManus B, Newman JW, Goodfriend T, Wishart DS (2011) The human serum metabolome. PLoS ONE 6:e16957

    Article  CAS  Google Scholar 

  • Rauch C, Feifel E, Amann E-M, Spötl HP, Schennach H, Pfaller W, Gstraunthaler G (2011) Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX 28:305–316

    Google Scholar 

  • Shailer C, Corrin K (1999) Serum supply: policies and controls operating in New Zealand. Dev Biol Stand 99:71–77

    CAS  Google Scholar 

  • van der Valk J, Mellor D, Brands R, Fischer R, Gruber F, Gstraunthaler G, Hellebrekers L, Hyllner J, Jonker FH, Prieto P, Thalen M, Baumans V (2004) The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol In Vitro 18:1–12

    Article  Google Scholar 

  • van der Valk J, Brunner D, De Smet K, Fex Svenningsen Å, Honegger P, Knudsen LE, Lindl T, Noraberg J, Price A, Scarino ML, Gstraunthaler G (2010) Optimization of chemically defined cell culture media—replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro 24:1053–1063

    Article  Google Scholar 

  • Wessmann SJ, Levings RL (1999) Benefits and risks due to animal serum used in cell culture production. Dev Biol Stand 99:3–8

    Google Scholar 

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Authors and Affiliations

  1. Division of Physiology, Innsbruck Medical University, Innsbruck, Austria

    Gerhard Gstraunthaler

  2. Institut für Angewandte Zellkultur, Munich, Germany

    Toni Lindl

  3. 3Rs—Center Utrecht Life Sciences, Utrecht University, Utrecht, The Netherlands

    Jan van der Valk

Authors
  1. Gerhard Gstraunthaler
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  2. Toni Lindl
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  3. Jan van der Valk
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Corresponding author

Correspondence to Gerhard Gstraunthaler.

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Gstraunthaler, G., Lindl, T. & van der Valk, J. A plea to reduce or replace fetal bovine serum in cell culture media. Cytotechnology 65, 791–793 (2013). https://doi.org/10.1007/s10616-013-9633-8

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  • DOI: https://doi.org/10.1007/s10616-013-9633-8

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