Abstract
‘Gold Veined Oxalis’ (Oxalis debilis) is an ornamental plant cultivar with attractive yellow vein foliage. After grafting scions of this plant to O. debilis plants that lacked the yellow vein foliage, grafted plants developed yellow vein suggesting that an infectious agent was the cause of the yellow vein. DNA extracts from O. debilis plants showing yellow vein were used in rolling circle amplification and PCR experiments. Sequence analyses of amplified DNA products suggested that a begomovirus was the cause of the yellow vein symptoms. The same begomovirus was found in the original sample and in graft inoculated plants that developed the yellow vein symptoms. The DNA A of the putative begomovirus, designated Oxalis yellow vein virus (OxYVV), shared 87 % overall nucleotide identities with the corresponding genome segments of Sida mottle virus, 85 % with Okra mottle virus, and 83 % with Tomato yellow spot virus. OxYVV virus was transmitted by the whitefly Bemisia tabaci Middle East-Asia Minor 1. This is another example of a virus that enhances the aesthetics of an ornamental plant.
Abbreviations
- AbMV:
-
Abutilon mosaic virus
- ClGMCNV:
-
Clerodendron golden mosaic China virus
- HYVMV:
-
Honeysuckle yellow vein mosaic virus
- HYVV:
-
Honeysuckle yellow vein virus
- OxYVV:
-
Oxalis yellow vein virus
- MEAM1:
-
Middle East-Asia Minor 1
- OMoV:
-
Okra mottle virus
- PCR:
-
Polymerase chain reaction
- RCA:
-
Rolling circle amplification
- SimMV:
-
Sida micrantha mosaic virus
- SiMBV:
-
Sida mosaic Brazil virus
- SiMoV:
-
Sida mottle virus
- TYLCV-IL:
-
Tomato yellow leaf curl virus-Israel
- ToYSV:
-
Tomato yellow spot virus
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Acknowledgments
The authors wish to thank Sead Sabanadzovic (Mississippi State University) and Andrea Hebert (Louisiana State University) for providing valuable suggestions to the manuscript. This research was partially supported by funds from the LSU AgCenter Zamorano Scholars Program, the Mississippi Agriculture and Forestry Experiment Station and the USDA National Institute of Food and Agriculture.
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Sequence reported in this paper has been deposited in the GenBank under accession number KM887907
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Herrera, F., Aboughanem-Sabanadzovic, N. & Valverde, R.A. A begomovirus associated with yellow vein symptoms of Oxalis debilis . Eur J Plant Pathol 142, 203–208 (2015). https://doi.org/10.1007/s10658-015-0594-y
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DOI: https://doi.org/10.1007/s10658-015-0594-y