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Genetic and phenotypic differences of Fusarium oxysporum f. sp. citri isolated from sweet orange and tangerine

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Abstract

Fusarium oxysporum f. sp citri is a new soil-borne pathogen of mature citrus trees in Tunisia. ISSR and RAPD markers were used in this study to characterize strains of Fusarium oxysporum from citrus in Tunisia. Pathogenicity assays revealed that Fusarium oxysporum isolates caused from 32 to 95 % disease on seedlings of Citrus aurantium. Results showed that pathogenic isolates were classified into two groups on the basis of ISSR and RAPD fingerprints, suggesting a level of genetic specificity and diversity not previously identified on Fusarium oxysporum f. sp citri. Cluster analysis, principal coordinate analysis and population structure indicated that the different isolates appears to be related only to the host. Potential primers related to ISSR markers could be selected to characterize Tunisian strains of Fusarium oxysporum and determine genetic differences of the pathogen virulence. With this investigation we characterize Fusarium wilt on citrus trees by the mean of ISSR and RAPD markers.

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Acknowledgments

The financial assistance provided by the Department of Science and Technology (DST) and Ministry of External Affairs (MEA), Government of India through the Federation of Indian Chambers of Commerce and Industry (FICCI), has launched the CV Raman Iternational Fellowship programme, Project Director of Derectorate Oilseed Research (DOR) Rajendranagar, Hyderabad. The authors are highly grateful and obliged to Dr D. Kumar, Biotechnology Lab, DOR and thanks Mr. J. Ramyya for the technical guidance.

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Authors and Affiliations

  1. Laboratory of Phytopathology, National Institute of Agronomy of Tunisia, Av Charles Nicolle 43, 1082, Tunis, Tunisia

    Ibtissem Hannachi & Mohamed Cherif

  2. DBIOS - Department of Life Science and Systems Biology, University of Torino, Viale Mattioli 25, 10125, Torino, Italy

    Anna Poli

  3. Department of Agronomy and Biotechnology, National Agronomic Institute of Tunisia, Av Charles Nicolle 43, 1082, Tunis, Tunisia

    Salah Rezgui

  4. Laboratory of Plant Pathology, Directorate of Oilseed Research, Rajendranagar, Hyderabad, 500 030, India

    Ruvalpali Durga Prassad

  5. Institute National Agronomique de Tunis, Université de Carthage, Avenue Charles Nicolle, 1082, Cité Mahrajène, Tunisie

    Ibtissem Hannachi

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  1. Ibtissem Hannachi
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  2. Anna Poli
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Correspondence to Ibtissem Hannachi.

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Hannachi, I., Poli, A., Rezgui, S. et al. Genetic and phenotypic differences of Fusarium oxysporum f. sp. citri isolated from sweet orange and tangerine. Eur J Plant Pathol 142, 269–280 (2015). https://doi.org/10.1007/s10658-015-0611-1

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  • DOI: https://doi.org/10.1007/s10658-015-0611-1

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