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Similar infection process and induced defense patterns during compatible interactions between Zymoseptoria tritici and both bread and durum wheat species

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Abstract

Bread wheat (BW) and durum wheat (DW) are both strongly affected by Septoria tritici blotch caused by the hemibiotrophic fungus Zymoseptoria tritici. However, only the BW-Z. tritici pathosystem has been well studied so far. Here, we compared compatible interactions between Z. tritici and both BW and DW species at the cytological, biochemical and molecular levels. Fungal infection process investigations showed close spore germination and leaf penetration features in both interactions, although differences in the patterns of these events were observed. During the necrotrophic phase, disease severity and sporulation levels were associated in both interactions with increases of the two cell-wall degrading enzyme activities endo-β-1,4-xylanase and endo-β-1,3-glucanase as well as protease. An analysis of plant defense responses during the first five days post inoculation revealed inductions of GLUC, Chi4, POX and PAL and a repression of LOX gene expressions in both wheat species, although differences in kinetics and levels of induction or repression were observed. In addition, peroxidase, catalase, glucanase, phenylalanine ammonia-lyase and lipoxygenase activities were induced in both wheat species, while only weak accumulations of hydrogen peroxide and polyphenols were detected at the fungal penetration sites. Our study revealed overall a similarity in Z. tritici infection process and triggered wheat defense pathways on both pathosystems.

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Acknowledgment

Lamia Somai-Jemmali was supported by Campus France for internships in Institut Supérieur d’Agriculture (Lille, France) and Université du Littoral Côte d’Opale (Calais, France).

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Authors and Affiliations

  1. Laboratory of Genetics and Plant Breeding, National Agronomic Institute of Tunis, 43 Avenue Charles Nicolle, 1082, Tunis, Tunisia

    Lamia Somai-Jemmali & Walid Hamada

  2. Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), GIS PhyNoPi, Université du Littoral Côte d’Opale, Université Lille-Nord de France, 50 Rue Ferdinand Buisson, C.S. 80699, F-62228, Calais cedex, France

    Béatrice Randoux, Maryline Magnin-Robert & Philippe Reignault

  3. Equipe Biotechnologie et Gestion des Agents Pathogènes en agriculture, Laboratoire Charles Viollette, GIS PhyNoPi, Institut Supérieur d’Agriculture, Université Lille-Nord de France, 48 Boulevard Vauban, F-59046, Lille cedex, France

    Ali Siah & Patrice Halama

  4. Higher School of Agriculture of Kef (ESAK), Boulifa 7119, Kef, Tunisia

    Walid Hamada

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  1. Lamia Somai-Jemmali
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  2. Béatrice Randoux
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Correspondence to Lamia Somai-Jemmali.

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Somai-Jemmali, L., Randoux, B., Siah, A. et al. Similar infection process and induced defense patterns during compatible interactions between Zymoseptoria tritici and both bread and durum wheat species. Eur J Plant Pathol 147, 787–801 (2017). https://doi.org/10.1007/s10658-016-1043-2

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  • DOI: https://doi.org/10.1007/s10658-016-1043-2

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