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Feed deprivation in Senegalese sole (Solea senegalensis Kaup, 1858) juveniles: effects on blood plasma metabolites and free amino acid levels

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Abstract

The effects of prolonged feed deprivation were assessed on blood plasma metabolites and free amino acid levels in Solea senegalensis. Juvenile specimens were maintained at two experimental conditions (24 h fasted and 21 days feed-deprived). In feed-deprived fish, relative growth rate and hepatosomatic index as well as plasma triglycerides and proteins levels were significantly lower. However, plasma cortisol levels were higher in feed-deprived fish, while plasma glucose and lactate values were not significantly different between treatments. Furthermore, feed-deprived fish showed higher levels of total plasma free amino acids than 24 h fasted fish. In 21 days feed-deprived sole, an increase in plasma cortisol levels may suggest a functional role in mobilizing energy due to the catabolic action of this hormone in teleosts. Higher levels of glutamine, arginine and ornithine in 21 days feed-deprived fish may be indicative of a dual role for these amino acids: ammonia detoxification and carbon source for gluconeogenesis. The increased plasma glucogenic and branched-chain amino acid levels, together with the maintenance of plasma glucose in 21 days feed-deprived sole, suggest active liver gluconeogenic processes supported by tissue proteolysis.

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Acknowledgments

This study was supported by projects STRESSAA—POCTI/CVT/49324/2002 (FCT, Portugal and FEDER), AGL2007-61211/ACU (Ministerio de Educación y Ciencia, Spain), and Proyecto de Excelencia PO7-RNM-02843 (Consejería de Innovación, Ciencia y Empresa. Junta de Andalucía) to J.M.M. Benjamín Costas and Cláudia Aragão were supported by Fundação para a Ciência e Tecnologia, Portugal (SFRH/BD/38697/2007 and SFRH/BPD/37197/2007, respectively).

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Authors and Affiliations

  1. CIMAR/CCMAR—Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    Benjamín Costas, Cláudia Aragão, Maria Teresa Dinis & Luís E. C. Conceição

  2. CIMAR/CIIMAR—Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal

    Benjamín Costas

  3. ICBAS—Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal

    Benjamín Costas

  4. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad Cádiz, Puerto Real, 11510, Cádiz, España

    Ignacio Ruiz-Jarabo, Luis Vargas-Chacoff, Francisco Jesús Arjona & Juan Miguel Mancera

  5. Instituto de Zoología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile

    Luis Vargas-Chacoff

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  1. Benjamín Costas
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Correspondence to Benjamín Costas.

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Costas, B., Aragão, C., Ruiz-Jarabo, I. et al. Feed deprivation in Senegalese sole (Solea senegalensis Kaup, 1858) juveniles: effects on blood plasma metabolites and free amino acid levels. Fish Physiol Biochem 37, 495–504 (2011). https://doi.org/10.1007/s10695-010-9451-2

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