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Low expression of Neu2 sialidase in the thymus of SM/J mice—existence of neuraminidase positive cells “Neu-medullocyte” in the murine thymus

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Abstract

We have already reported that the homogenate of the A/J mouse thymus shows a high sialidase activity at the neutral pH region and that in both soluble and membrane fractions optimal pH was 6.5–7 (Kijimoto-Ochiai et al., Glycoconj. J., 20:375–384, 2004). In the present study, we investigated the level of sialidase activities in the thymus of the SM/J mouse, a mouse strain that we know to have a Neu1a allele that reveals a low level of sialidase activity in the liver. We found that while in the A/J thymus the soluble sialidase activity at pH 6.5 was high, the SM/J thymus lacked all such activity. A QTL analysis of SMXA recombinant inbred strains showed that soluble sialidase activity correlated well with the D1Mit8/9 marker on chromosome 1. The murine whole DNA-sequence data and the results of our FISH analysis (Kotani et al., Biochem. Biophys. Res. Comm., 286:250–258, 2001) showed that this location is consistent with the position of Neu2 gene. We confirmed that it is hard to detect the Neu2 enzyme of the SM/J mouse thymus by an anti-Neu2 antibody using a Western blot analysis. We also found that while the mRNA expression of Neu2 was quite normal in the SM/J mouse liver, it was very low in the SM/J mouse thymus. We therefore conclude that the lack of soluble sialidase activity in the SM/J mouse thymus is due to the thymus-specific low expression level of the Neu2 gene. We have previously shown that the sialidase positive cell which contains the Mac-1 and immunoglobulin, and which is located sparsely in the corticomedullar region or medullary region of the A/J mouse thymus (Kijimoto-Ochiai et al., Glycoconj. J., 20:375–384, 2004). We showed now in this paper that the detection of this cell in the SM/J mouse thymus at pH 7.0 was difficult. We propose, therefore, to name the cell “Neu-medullocyte”.

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Acknowledgment

This study started at the Institute of Immunology/Institute for genetic medicine Hokkaido University when S.K-O was there (H.M., M.F., and K.T. were also there) under the support of a Grant-in-Aid for Scientific Research (C)(2)(07808069) from the Ministry of Education of Japan (1995–1996). Supply of the SM/J, SMXA mice and QTL analysis were done by MN. RT-PCR analysis was done by TK and TS. We are very grateful to Drs. Norimasa Iwasaki and Yuichiro Abe for kind permission to use the real time PCR equipment, and also to Dr. Taeko. Miyagi (Cancer Institute of Miyagi, Japan) for kind gift of anti-Neu2 polyclonal antibody. We also thank Ms. S. Yoshida and Prof. Y. Igarashi (Hokkaido U.), and Ms M. Ishii and Prof. I. Matsuoka (Hokkaido U.) for supporting to use the facilities in ECL-Western blot analysis and in FACS analysis, respectively. The author(s.K-O) would appreciate very much Professor Dr. S. Hakomori (Pacific Northwest Research Institute, and Department of Pathology and Microbiology, University of Washington, Seattle, WA 98122-4327) suggesting to name the new type of sialidase-positive cell when it was published in 2004 (Glycoconjugate J. 20, 375-384). Encouraged by his suggestion, the author named it as “Neu-medullocyte” in this paper, and would like to acknowledge it.

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Author notes

  1. S. Kijimoto-Ochiai

    Present address: Graduate School of Advanced Life Science, Hokkaido University, Kita-21, Nishi-11, Kita-ku, Sapporo, 001-0021, Japan

  2. H. Matsukawa

    Present address: , Moegino 6-9-303, Aoba-ku, Yokohama, 227-0044, Japan

  3. M. Fujii

    Present address: Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan

  4. K. Tomobe

    Present address: Yokohama University of Pharmacy, Yokohama, Japan

  5. M. Nishimura

    Present address: Department of Medical Technology, Nagoya University School of Health Science, 1-1-20 Diko-Minami, Higashi-ku, Nagoya, 461-8673, Japan

Authors and Affiliations

  1. Institute of Immunology/Genetic Medicine, Hokkaido University, N15, W7, Sapporo, 060-0815, Japan

    S. Kijimoto-Ochiai, H. Matsukawa, M. Fujii & K. Tomobe

  2. Graduate School of Advanced Life Science, Hokkaido University, Kita-21, Nishi-11, Kita-ku, Sapporo, 001-0021, Japan

    T. Koda & T. Suwama

  3. Institute for Laboratory Animal Research, Nagoya University School of Medicine, Nagoya, 466-850, Japan

    M. Nishimura

  4. Creative Research Initiative “Sousei”, Hokkaido University, Kita-21, Nishi-10, Kita-ku, Sapporo, 001-0021, Japan

    S. Kijimoto-Ochiai

  5. Life Space Cosmos, Kita-3, Nishi-13, 3-12, Chuo-ku, Sapporo, 060-0003, Japan

    S. Kijimoto-Ochiai

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  1. S. Kijimoto-Ochiai
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Correspondence to S. Kijimoto-Ochiai.

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Kijimoto-Ochiai, S., Koda, T., Suwama, T. et al. Low expression of Neu2 sialidase in the thymus of SM/J mice—existence of neuraminidase positive cells “Neu-medullocyte” in the murine thymus. Glycoconj J 25, 787–796 (2008). https://doi.org/10.1007/s10719-008-9126-8

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