Abstract
Using recombinant tetanus toxin HC fragment (rTT-HC) as carrier, we prepared multimeric bivalent immunogens featuring the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Ogawa, in combination with either the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Inaba, or a synthetic disaccharide tetrapeptide peptidoglycan fragment as adjuvant. The conjugation reaction was effected by squaric acid chemistry and monitored in virtually real time by SELDI-TOF MS. In this way, we could prepare well-defined immunogens with predictable carbohydrate–carrier ratio, whose molecular mass and the amount of each saccharide attached could be independently determined. The ability to prepare such neoglycoconjugates opens unprecedented possibilities for preparation of conjugate vaccines for bacterial diseases from synthetic carbohydrates.
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Abbreviations
- TT:
-
Tetanus toxoid
- rTT-HC :
-
Recombinant tetanus toxin fragment C
- HC :
-
Tetanus toxin fragment C
- O-PS:
-
O-specific Polysaccharides
- SELDI-TOF-MS:
-
Surface-enhanced laser desorption time-of-flight mass spectrometry
- BSA:
-
Bovine serum albumin
- NIS:
-
N-Iodosuccinimide
- PG:
-
Peptidoglycan
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This research was supported by the Intramural Research Program of the NIH, NIDDK.
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Bongat, A.F.G., Saksena, R., Adamo, R. et al. Multimeric bivalent immunogens from recombinant tetanus toxin HC fragment, synthetic hexasaccharides, and a glycopeptide adjuvant. Glycoconj J 27, 69–77 (2010). https://doi.org/10.1007/s10719-009-9259-4
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DOI: https://doi.org/10.1007/s10719-009-9259-4