Abstract
Polygonatum sibiricum (P. sibiricum) rhizomes are widely used as a tonic and functional food, and are often processed to enhance their tonic function by repeated steaming and drying. As the most important constituent, the polysaccharide from P. sibiricum rhizomes (PSP) has demonstrated various activities, but the alteration of structural characteristics and activities of the purified PSPs during steaming process was rarely investigated. To well understand the effect of steaming process on the polysaccharides of P. sibiricum, neutral polysaccharides from P. sibiricum rhizomes (PSP0 ~ PSP9) after steaming were first isolated and purified, and then the chemical properties and antioxidant activities were determined. The results showed that the molar ratios of monosaccharides in PSPs were different. The molecular weights of PSPs were increased significantly after the fourth steaming. Morphological studies showed that the surface of PSPs became much tighter during the steaming process. Fourier transform infrared spectroscopy spectra displayed the polysaccharides had similar backbones and chemical groups. Furthermore, the antioxidant activity of PSPs was measured through radical scavenging tests. It was found that the radical scavenging activity of PSPs was elevated strikingly after steaming, and increased gradually with numbers of steaming process. The biological and chemical variance of PSPs revealed considerable segregation of PSP0, PSP1 ~ PSP4 and PSP5 ~ PSP9. In conclusion, our results proposed the fourth time as the optimal number of steaming to extract functional polysaccharide from P. sibiricum rhizomes.
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Zhao, P., Zhao, C., Li, X., Gao, Q., Huang, L., Xiao, P., Gao, W.: The genus Polygonatum: A review of ethnopharmacology, phytochemistry and pharmacology. J. Ethnopharmacol. 214, 274–291 (2018)
Wujisguleng, W., Liu, Y., Long, C.: Ethnobotanical review of food uses of Polygonatum (Convallariaceae) in China. Acta Soc. Bot. Pol. 81, 239–244 (2012)
Zhao, H., Wang, Q. L., Hou, S. B., Chen, G.: Chemical constituents from the rhizomes of Polygonatum sibiricum Red. and anti-inflammatory activity in RAW264.7 macrophage cells. Nat. Prod. Res. 33, 2359–2362 (2018)
Wang, J., Lu, C.S., Liu, D.Y., Xu, Y.T., Wu, H.H.: Constituents from Polygonatum sibiricum and their inhibitions on the formation of advanced glycosylation end products. J. Asian Nat. Prod. Res. 18, 697–704 (2016)
Long, T., Liu, Z., Shang, J., Zhou, X., Yu, S., Tian, H., Bao, Y.: Polygonatum sibiricum polysaccharides play anti-cancer effect through TLR4-MAPK/NF-κB signaling pathways. Int. J. Biol. Macromol. 111, 813–821 (2018)
Cui, X., Wang, S., Hui, C., Hong, G., Li, Y., Xu, F., Zheng, M., Xi, X., Han, C.: A Review: The Bioactivities and Pharmacological Applications of Polygonatum sibiricum polysaccharides. Molecules 23, 1170–1181 (2018)
Liu, Z., Ni, H., Yu, L., Xu, S., Wang, D.: Adjuvant activities of CTAB-modified Polygonatum sibiricum polysaccharide cubosomes on immune responses to ovalbumin in mice. Int. J. Biol. Macromol. 148, 793–801 (2020)
Li, L., Thakur, K., Cao, Y., Liao, B., Zhang, J., Wei, Z.: Anticancerous potential of polysaccharides sequentially extracted from Polygonatum cyrtonema Hua in Human cervical cancer Hela cells. Int. J. Biol. Macromol. 148, 843–850 (2020)
Cheng, R., Liao, X., Addou, A.M., Qian, J., Wang, S., Cheng, Z., Wang, L., Huang, J.: Effects of “nine steaming nine sun-drying” on proximate composition, oil properties and volatile compounds of black sesame seeds. Food Chem. 344, 128577–128585 (2021)
Yoo, S., Park, B.I., Kim, D.H., Lee, S., Lee, S.H., Shim, W.S., Seo, Y.K., Kang, K., Lee, K.T., Yim, S.V., Soung, D.Y., Kim, B.H.: Ginsenoside absorption rate and extent enhancement of black ginseng (CJ EnerG) over red ginseng in healthy adults. Pharmaceutics. 13, 487–501 (2021)
Gong, P.Y., Guo, Y.J., Tian, Y.S., Gu, L.F., Qi, J., Yu, B.Y.: Reverse tracing anti-thrombotic active ingredients from dried Rehmannia Radix based on multidimensional spectrum-effect relationship analysis of steaming and drying for nine cycles. J. Ethnopharmacol. 276, 114177–114186 (2021)
Jin, J., Lao, J., Zhou, R., He, W., Qin, Y., Zhong, C., Xie, J., Liu, H., Wan, D., Zhang, S., Qin, Y.: Simultaneous identification and dynamic analysis of saccharides during steam processing of rhizomes of Polygonatum cyrtonema by HPLC–QTOF–MS/MS. Molecules 23, 2855–2867 (2018)
Xu, Z., Wang, H., Wang, B., Fu, L., Yuan, M., Liu, J., Zhou, L., Ding, C.: Characterization and antioxidant activities of polysaccharides from the leaves of Lilium lancifolium Thunb. Int. J. Biol. Macromol. 92, 148–155 (2016)
Fan, L., Li, J., Deng, K., Ai, L.: Effects of drying methods on the antioxidant activities of polysaccharides extracted from Ganoderma lucidum. Carbohyd. Polym. 87, 1849–1854 (2012)
Chen, H.M., Yan, X.J.: Antioxidant activities of agaro-oligosaccharides with different degrees of polymerization in cell-based system. Biochem. Biophys. Acta. 1722, 103–111 (2005)
Zhang, J., Liu, N., Sun, C., Sun, D., Wang, Y.: Polysaccharides from Polygonatum sibiricum Delar. ex Redoute induce an immune response in the RAW264.7 cell line via an NF-κB/MAPK pathway. Rsc Adv. 9, 17988–17994 (2019)
Zhao, P., Li, X., Wang, Y., Yan, L., Guo, L., Huang, L., Gao, W.: Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp. Carbohyd. Polym. 233, 115836–115843 (2020)
Liu, X., Wan, Z., Shi, L., Lu, X.: Preparation and antiherpetic activities of chemically modified polysaccharides from Polygonatum cyrtonema Hua. Carbohyd. Polym. 83, 737–742 (2011)
Dubois, M., Gilles, K., Hamilton, J., Rebers, P., Smith, F.: A colorimetric method for the determination of sugars. Nature 168, 167 (1951)
Zimm, Bruno, H.: The scattering of light and the radial distribution function of high polymer solutions. J. Chem. Phys. 16, 1093–1099 (1948)
Mukherjee, A., Dumont, M., Cherestes, A.: Production of 5-Hydroxymethylfurfural from starch through an environmentally-friendly synthesis pathway. Catal. Lett. 149, 283–291 (2019)
Chen, S., Xu, J., Xue, C., Dong, P., Sheng, W., Yu, G., Chai, W.: Sequence determination of a non-sulfated glycosaminoglycan-like polysaccharide from melanin-free ink of the squid Ommastrephes bartrami by negative-ion electrospray tandem mass spectrometry and NMR spectroscopy. Glycoconjugate J. 25, 481–492 (2008)
Fu, L., Chen, H., Dong, P., Zhang, X., Zhang, M.: Effects of ultrasonic treatment on the physicochemical properties and DPPH radical scavenging activity of polysaccharides from mushroom Inonotus obliquus. J. Food Sci. 75, 322–327 (2010)
Zhou, H.C., Lin, Y.M., Li, Y.Y., Li, M., Wei, S.D., Chai, W.M., Tam, F.Y.: Antioxidant properties of polymeric proanthocyanidins from fruit stones and pericarps of Litchi chinensis Sonn. Food Res. Int. 44, 613–620 (2011)
Jen, J., Leu, M.F., Yang, T.: Determination of hydroxyl radicals in an advanced oxidation process with salicylic acid trapping and liquid chromatography. J. Chromatogr. A. 796, 283–288 (1998)
Jin, Y., Kim, Y., Jeon, J., Wang, C., Min, J., Noh, H., Yang, D.: Effect of white, red and black ginseng on physicochemical properties and ginsenosides. Plant Food. Hum. Nutr. 70, 141–145 (2015)
Shin, J., Park, Y.J., Kim, W., Kim, D., Kim, B., Lee, H., Baik, M.: Change of ginsenoside profiles in processed ginseng by drying, steaming, and puffing. J. Microbiol. Biotechn. 29, 222–229 (2019)
Yu, H., Seow, Y., Ong, P.K.C., Zhou, W.: Kinetic study of high-intensity ultrasound-assisted Maillard reaction in a model system of β-glucose and glycine. Food Chem. 269, 628–637 (2018)
Capuano, E., Ferrigno, A., Acampa, I., Serpen, A., Acar, O.C., Goekmen, V., Fogliano, V.: Effect of flour type on Maillard reaction and acrylamide formation during toasting of bread crisp model systems and mitigation strategies. Food Res. Int. 42, 1295–1302 (2009)
Zhao, P., Zhou, H., Zhao, C., Li, X., Gao, W.: Purification, characterization and immunomodulatory activity of fructans from Polygonatum odoratum and P. cyrtonema. Carbohyd. Polym. 214, 44–52 (2019)
Wang, Y., Liu, N., Xue, X., Li, Q., Sun, D., Zhao, Z.: Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum. Int. J. Biol. Macromol. 160, 688–694 (2020)
Yelithao, K., Surayot, U., Lee, J. H., You, S.: RAW264.7 Cell activating glucomannans extracted from rhizome of Polygonatum sibiricum. Prev Nutr Food Sci. 21, 245–254 (2016)
Li, N., Shi, C., Shi, S., Wang, H., Yan, J., Wang, S.: An inulin-type fructan isolated from Artemisia japonica and its anti-arthritic effects. J. Funct Foods. 29, 29–36 (2017)
Fu, Y., Feng, K., Wei, S., Xiang, X., Wu, D.: Comparison of structural characteristics and bioactivities of polysaccharides from loquat leaves prepared by different drying techniques. Int. J. Biol. Macromol. 145, 611–619 (2020)
Chen, G., Li, C., Wang, S., Mei, X., Zhang, H., Kan, J.: Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures. Food Chem. 292, 281–293 (2019)
Chen, X., Xu, X., Zhang, L., Zeng, F.: Chain conformation and anti-tumor activities of phosphorylated (1→3)-β-d-glucan from Poria cocos. Carbohyd. Polym. 78, 581–587 (2009)
Chen, C., Wu, W., Xu, X., Zhang, L., Liu, Y., Wang, K.: Chain conformation and anti-tumor activity of derivatives of polysaccharide from Rhizoma Panacis Japonici. Carbohyd. Polym. 105, 308–316 (2014)
Xu, S., Xu, X., Zhang, L.: Branching structure and chain conformation of water-soluble glucan extracted from Auricularia auricula-judae. J. Agr. Food Chem. 60, 3498–3506 (2012)
Bahrani, S., Loisel, C., Rezzoug, S., Cohendoz, S., Buleon, A., Maache-Rezzoug, Z.: Physicochemical and crystalline properties of standard maize starch hydrothermally treated by direct steaming. Carbohyd. Polym. 157, 380–390 (2017)
Li, Q., Chen, J., Huyan, Z., Kou, Y., Xu, L., Yu, X., Gao, J.M.: Application of fourier transform infrared spectroscopy for the quality and safety analysis of fats and oils: A review. Crit. Rev. Food Sci. 59, 3597–3611 (2019)
Chen, Z.E., Wufuer, R., Ji, J.H., Li, J.F., Cheng, Y.F., Dong, C., Taoerdahong, H.: Structural characterization and immunostimulatory activity of polysaccharides from Brassica rapa L. J. Agric. Food Chem. 65, 9685–9692 (2017)
Liu, Y., Luo, M., Liu, F., Feng, X., Ibrahim, S.A., Cheng, L., Huang, W.: Effects of freeze drying and hot-air drying on the physicochemical properties and bioactivities of polysaccharides from Lentinula edodes. Int. J. Biol. Macromol. 145, 476–483 (2020)
Acknowledgements
This work was supported by Grants from Natural Science Foundation of Shandong Province (ZR2020QB164), Research and Innovation Fund of Weihai Scince and Technology Development Plan Project, Natural Science Foundation of Shandong Province (ZR2019MB009).
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Li, Q., Zeng, J., Gong, P. et al. Effect of steaming process on the structural characteristics and antioxidant activities of polysaccharides from Polygonatum sibiricum rhizomes. Glycoconj J 38, 561–572 (2021). https://doi.org/10.1007/s10719-021-10013-z
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DOI: https://doi.org/10.1007/s10719-021-10013-z