Abstract
Fifty-three wheat cultivars have been genotyped using 24 SSR (simple sequence repeat) markers in order to evaluate genetic similarities among Polish wheats, i.e. 53 spring and winter cultivars; ‘Chinese Spring’ was taken as reference. ll but one SSR marker allowed to identify DNA polymorphisms, giving in total 166 alleles (including nulls), from 3 to 13 alleles per marker with mean of 7.22. Based on marker data, genetic similarities were calculated and a dendrogram was created. ‘Spring’ cultivars were less diverse than winter ones, showing the biggest similarity to ‘Chinese Spring’. Four sister cultivars (Nutka, Tonacja, Zyta and Sukces), formed a cluster of very similar materials, of which Zyta and Sukces had the highest similarity indices. Parental lines Jubilatka and SMH 2182 were more distant from each other (genetic similarity of 0.227). It was possible to differentiate all the wheats using only four SSR markers: Xgwm186, Xgwm389, Xgwm459 and Xgwm577.
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Börner A, Röder MS, Unger O, Meinel A (2000) The detection and molecular mapping of a major gene for non-specific adult-plant disease resistance against stripe rust (Puccinia striiformis) in wheat. Theor Appl Genet 100:1095–1099
Burkhamer RL, Lanning SP, Martens RJ, Martin JM, Talbert LE (1998) Predicting progeny variance from parental divergence in hard red spring wheat. Crop Sci 38:243–248
Chen HB, Martin JM, Lavin M, Talbert LE (1994) Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers. Crop Sci 34:1628–1632
Devos KM, Bryan GJ, Collins AJ, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252
Donini P, Stephenson P, Bryan GJ, Koebner RMD (1998) The potential of microsatellites for high troughput genetic diversity assessment in wheat and barley. Genet Resour Crop Evol 45:415–421
Fahima T, Röder MS, Wendehake K, Kirzhner VM, Nevo E (2002) Microsatellite polymorphism in natural populations of wild emmer wheat, Triticum dicoccoides, in Israel. Theor Appl Genet 104:17–29
Feldman M (2001) Origin of cultivated wheat. In: Bonjean AP, Angus WJ (eds) The World wheat book. A history of wheat breeding. Intercept, Paris, pp 3–56
Ford R, Le Roux K, Itman C, Brouwer JB, Taylor PWJ (2002) Diversity analysis and genotyping in Pisum with sequence tagged microsatellite site (STMS) primers. Euphytica 124:397–405
Hammer K, Filatenko AA, Korzun V (2000) Microsatellite markers: a new tool for distinguishing diploid wheat species. Genet Resour Crop Evol 47:497–505
Harding RM, Boyce AJ, Clegg JB (1992) The evolution of tandemly repetitive DNA: recombination rules. Genetics 132:847–859
Huang XQ, Börner A, Röder MS, Ganal MW (2002) Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor Appl Genet 105:699–707
Huang XQ, Zeller FJ, Hsam SLK, Wenzel G, Mohler V (2000) Chromosomal location of AFLP markers in common wheat (Triticum aestivum L.) utilizing nulli-tetrasomic stocks. Genome 43:298–305
Irzykowska L, Żółtańska E, Bocianowski J (2005) Use of molecular and conventional techniques to identify and analyze genetic variability of Rhizoctonia spp. isolates. Acta Agrobot 59(2):19–32
Joshi CP, Nguyen HT (1993) RAPD (random amplified polymorphic DNA) analysis based intervarietal genetic relationships among hexaploid wheats. Plant Sci 93:95–103
Kim HS, Ward RW (2000) Patterns of RFLP-based genetic diversity in germplasm pools of common wheat with different geographical or breeding program origins. Euphytica 115:197–208
Kuczyńska A, Milczarski P, Surma M, Masojć P, Adamski T (2001) Genetic diversity among cultivars of spring barley revealed by random amplified polymorphic DNA (RAPD). J Appl Genet 42(1):43–48
Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76(10):5269–5273
Parker GD, Chalmers KJ, Rathjen AJ, Langride P (1998) Mapping loci associated with flour colour in wheat (Triticum aestivum L.). Theor Appl Genet 97:238–245
Peng JH, Fahima T, Röder MS, Li YC, Dahan A, Grama A, Ronin YI, Korol AB, Nevo E (1999) Microsatellite tagging of the stripe-rust resistance gene YrH52 derived from wild emmer wheat, Triticum dicoccoides, and suggestive negative crossover interference on chromosome 1B. Theor Appl Genet 98:862–872
Pestsova E, Korzun V, Goncharov NP, Hammer K, Ganal MW, Röder MS (2000) Microsatellite analysis of Aegilops tauschii germplasm. Theor Appl Genet 101:100–106
Plaschke J, Ganal MW, Röder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007
Prasad M, Varsney RK, Roy JK, Balyan HS, Gupta PK (2000) The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor Appl Genet 100:584–592
Rongwen J, Akkaya MS, Bhagwat AA, Lavi U, Cregan PB (1995) The use of microsatellite DNA markers for soybean genotype identification. Theor Appl Genet 90:43–48
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Röder MS, Wendehake K, Korzun V, Bredemeijer G, Laborie D, Bertrand L, Isaac P, Rendell S, Jackson J, Cooke RJ, Vosmann B, Ganal MW (2002) Construction and analysis of a microsatellite-based database of European wheat cultivars. Theor Appl Genet 106:67–73
Stachel M, Lelly T, Grausgruber H, Vollmann J (2000) Application of microsatellites in wheat (Triticum aestivum L.) for studying genetic differentiation caused by selection for adaptation and use. Theor Appl Genet 100:242–248
Stępień Ł, Golka L, Chełkowski J (2003) Leaf rust resistance genes of wheat: identification in cultivars and resistance sources. J Appl Genet 44:139–149
Tachida W, Iizuka M (1992) Persistence of repeated sequences that evolve by replication slippage. Genetics 131:471–478
Weber Z, Irzykowska L, Bocianowski J (2005) Analysis of mycelial growth rates and RAPD-PCR profiles in a population of Gaeumannomyces graminis var. tritici originating from wheat plants grown from fungicide-treated seed. J Phytopathol 153:318–324
Yashitola J, Thirumurugan T, Sundaram RM, Naseerullah MK, Ramesha MS, Sarma NP, Sonti RV (2002) Assessment of purity of rice hybrids using microsatellite and STS markers. Crop Sci 42:1369–1373
Acknowledgements
Authors would like to thank Dr. Marion Röder for the sequences of unpublished microsatellites. This research was supported by PBZ KBN 029/P06/2000/09 grant. Łukasz Stępień received a Foundation for Polish Science scholarship for the year 2005.
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Stępień, Ł., Mohler, V., Bocianowski, J. et al. Assessing genetic diversity of Polish wheat (Triticum aestivum) varieties using microsatellite markers. Genet Resour Crop Evol 54, 1499–1506 (2007). https://doi.org/10.1007/s10722-006-9140-2
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DOI: https://doi.org/10.1007/s10722-006-9140-2