Abstract
Forty-seven endogenous cytokinin metabolites were determined in samples of long-term in vitro multiple shoot cultures of pea (Pisum sativum L.). Significant differences were found in the levels of all cytokinin groups except cis-zeatin and some of its derivatives. Old cultures (30 or 10 years old) contained larger total and active cytokinin pools than freshly prepared cultures. Long-term shoot cultures maintained on cytokinin-supplemented media initially exhibit gradually increasing concentrations of endogenous cytokinins that subsequently stabilise at a high level, which can make it more difficult to induce rooting later on. This is consistent with previous findings concerning the growth and development of plantlets derived from long-term cultures and the generally accepted role of cytokinins during root development. The results presented herein demonstrate the utility of long-term pea cultures as model systems for studying the influence of long-term cultivation on genetic stability, and suggest ways of circumventing the rooting problems associated with such cultures.
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Abbreviations
- BAP:
-
6-Benzylaminopurine
- cZ:
-
cis-Zeatin
- DHZ:
-
Dihydrozeatin
- *9G:
-
9-β-d-Glucopyranosyl derivative
- *5′MP:
-
5′-Monophosphate derivative
- *OG:
-
O-β-d-Glucopyranosyl derivative
- tZ:
-
trans-Zeatin
- UHPLC–ESI(+)–MS/MS:
-
Ultra high-performance liquid chromatography–electrospray tandem mass spectrometry
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Acknowledgments
This work was supported by the Ministry of Education Youth and Sports, Czech Republic (Grant LO1204 from the National Program of Sustainability), by IGA of Palacký University (IGA_PrF_2014006) and by the Ministry of Agriculture of the Czech Republic project No. RO 1014. The authors would like to thank to Dave Richardson and Sees-editing Ltd. for careful reading and editing of this manuscript.
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Plačková, L., Hrdlička, J., Smýkalová, I. et al. Cytokinin profiling of long-term in vitro pea (Pisum sativum L.) shoot cultures. Plant Growth Regul 77, 125–132 (2015). https://doi.org/10.1007/s10725-015-0044-z
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DOI: https://doi.org/10.1007/s10725-015-0044-z