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Evaluation of seed quality based on changes of internal substances during tobacco seed (Nicotiana tabacum L.) development

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Abstract

Seed quality is closely associated with internal substances during seed development. Tobacco seed varieties Honghua Dajinyuan (HD) and Yunyan97 (Y97) had higher viability and vigor when harvested from 31 to 33 days after pollination (DAP), subsequently decline slightly on 35 DAP. From 7 to 35 DAP, the moisture content of HD and Y97 decreased sharply from 90 to 30%, and abscisic acid declined more than 80% totally in seeds. Accumulation of oil and protein was accompanied with the consumption of soluble sugar and starch, especially from 7 to 23 DAP. In addition, crude fiber and arginine exhibited an increasing trend, while ash and lysine significantly decreased from 7 to 23 DAP. Generally, the germination percentage (seed viability) in HD and Y97 seed was related to oil, moisture, soluble sugar, protein, starch, ABA, lysine, arginine, seed weight and seed coat color; however, only moisture, oil and protein had a significant correlation with vigor index (seed vigor). Thus, seed viability could be applied to estimate the relationship between internal substances and seed quality, and predict the optimum seed harvest time of tobacco.

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Abbreviations

HD:

Honghua Dajinyuan

Y97:

Yunyan97

DAP:

Days after pollination

GE:

Germination energy

GP:

Germination percentage

GI:

Germination index

VI:

Vigor index

DW:

Dry weight

ABA:

Abscisic acid

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Nos. 31201279, 31371708, 31671774), Zhejiang Provincial Natural Science Foundation (Nos. LY15C130002, LZ14C130002), Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University and Jiangsu Collaborative Innovation Center for Modern Crop Production, P. R. China. Meanwhile, we would like to express our gratitude to Yang Wang, Yuchan Zhang and Zhihao Zhang for performing the experiments.

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Authors and Affiliations

  1. Seed Science Center, Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Zhan Li, Fan Li, Genyuan Guo, Yue Gao, Ronghui Pan, Yajing Guan & Jin Hu

  2. Yunnan Academy of Tobacco Agricultural Sciences, Yuxi, 653100, People’s Republic of China

    Wenguang Ma

  3. Yuxi Zhongyan Tobacco Seed Company Ltd., Yuxi, 653100, People’s Republic of China

    Wenguang Ma

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  1. Zhan Li
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  2. Fan Li
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  5. Wenguang Ma
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Correspondence to Yajing Guan.

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Li, Z., Li, F., Guo, G. et al. Evaluation of seed quality based on changes of internal substances during tobacco seed (Nicotiana tabacum L.) development. Plant Growth Regul 86, 389–399 (2018). https://doi.org/10.1007/s10725-018-0437-x

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  • DOI: https://doi.org/10.1007/s10725-018-0437-x

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