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Expression patterns of imprinted gene Inpp5f-v3 during mouse brain development

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Abstract

Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis revealed that only one transcript approx 2.7 kb of Inpp5f-v3 was detected in brain. The signals were only observed in brain by the whole-mount in situ hybridization at embryonic day 11.5 (E11.5). The results of quantitative real-time PCR (QRT-PCR) showed that the expression of Inpp5f-v3 increased gradually from the E11.5 to E17.5 and reached the highest at E17.5, then decreased at E18.5 during the brain development. Inpp5f-v3 gene was strongly expressed in the cerebral cortex, olfactory bulb, external germinal layer of cerebellum and ventricular zone (Vz) during the embryonic development (E15.5–E19.5), whereas the expression increased in the olfactory bulb and the cerebellum after birth by using in situ hybridization. The results also demonstrated that the expression of Inpp5f-v3 gene mainly located in olfactory bulb and hippocampus at postnatal day 7 (P7) and adulthood. These results suggest that Inpp5f-v3 is specifically expressed in mouse brain, and may function in the development of mouse brain.

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Acknowledgments

This work was supported from the National Natural Science Foundation of China (No. 30971645) and Heilongjiang Province Technological Project Program Returning Foundation (No. LC08C05).

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Authors and Affiliations

  1. Department of Life Science and Engineering, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, 150001, Heilongjian, China

    Chen Yan, He Hongjuan, Xing Yanjiang, Han Zhengbin, Li Kai, Zhang Fengwei, Hou Jing & Wu Qiong

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  1. Chen Yan
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  2. He Hongjuan
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  3. Xing Yanjiang
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  4. Han Zhengbin
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  5. Li Kai
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  6. Zhang Fengwei
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  7. Hou Jing
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  8. Wu Qiong
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Correspondence to Wu Qiong.

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Yan, C., Hongjuan, H., Yanjiang, X. et al. Expression patterns of imprinted gene Inpp5f-v3 during mouse brain development. J Mol Hist 42, 167–173 (2011). https://doi.org/10.1007/s10735-011-9321-y

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  • DOI: https://doi.org/10.1007/s10735-011-9321-y

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