Abstract
Follistain-like protein 1 (FSTL1), has been recently demonstrated to be involved in the embryo development of nervous system and glioblastoma. However, the role of FSTL1 in neuroinflammation remains unexplored. In this study, the expression of FSTL1 in astrocytes was verified and its role was studied in neuroinflammation induced by in vivo intracerebroventricular (ICV) injection of lipopolysaccharide (LPS) or LPS treatment to astrocytes in vitro. FSTL1 was significantly induced after ICV LPS injection or LPS treatment. FSTL1 suppressed upregulation of pro-inflammatory cytokines in astrocytes after LPS treatment. Moreover, FSTL1 downregulated expression of pro-inflammatory cytokines through suppressing MAPK/p-ERK1/2 pathway in astrocytes. Our results suggest that FSTL1 may play an anti-inflammatory role in neuroinflammation mediated by astrocytes.
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Abbreviations
- FSTL1:
-
Follistatin-like protein 1
- hrFSTL1:
-
Human recombined Follistatin-like protein 1
- LPS:
-
Lipopolysaccharide
- ICV:
-
Intracerebroventricular
- N.S.:
-
Normal saline
- CNS:
-
Central nervous system
- HIF:
-
Hypoxia induced factor
- TNF-α:
-
Tumor necrosis factor alpha
- IL-1β:
-
Interleukin 1 beta
- IL-6:
-
Interleukin 6
- COX2:
-
Cyclooxygenase-2
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Acknowledgements
We thank Chong-Bo Yang for his patient review and critical thinking of our manuscript. This work was supported by the General Program of National Natural Science Foundation of China (Grant No. 81572191).
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Kai-Yuan Cheng and Yi Liu have contributed equally to this work.
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Cheng, KY., Liu, Y., Han, YG. et al. Follistatin-like protein 1 suppressed pro-inflammatory cytokines expression during neuroinflammation induced by lipopolysaccharide. J Mol Hist 48, 63–72 (2017). https://doi.org/10.1007/s10735-016-9706-z
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DOI: https://doi.org/10.1007/s10735-016-9706-z