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Morphometry and growth of three Synechococcus-like picoplanktic cyanobacteria at different culture conditions

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Abstract

Three phycocyanin-rich strains of Synechococcus-like picoplanktic cyanobacteria, isolated from the plankton of Czech oligotrophic to eutrophic freshwater reservoirs, were investigated in crossed gradients of light and temperature and in combination with two different culture media (BG-11 and WC). The strains exhibited similar growth and reproduction patterns and displayed overlapping ranges of cell size (1.5 × 0.8 μm) under standardized laboratory conditions (18 μmol m−2 s−1; 20°C). However, strains behavior differed in the crossed gradients. All strains preferred BG-11 medium, where also remarkable size changes could be observed. Length, width, cell abundance and growth rate of two strains were positively correlated with temperature and nutrients, whereas the impact of light intensity was insignificant. Maximum cell elongation (involution cells up to 19 μm) occurred in two strains only in BG-11 medium at highest temperature (28°C) and highest irradiance (53 μmol m−2 s−1). Cell dimensions in WC medium were constant under most conditions given. The third strain was influenced by all three factors, from which light and nutrients played pivotal role. The length of the lag-phase for all strains appeared to be temperature dependent (negative correlation). Despite the fact that the cell volume in all strains increased more than five times under the lowest light and low temperature (6 μmol m−2 s−1, <15°C) in both media, the length/width ratio remained unchanged. The strains differed in the degree of cell enlargement and cell division symmetry as well as in optimum temperature and light dependence. Based on this experimental work two strains could be identified as Synechococcus sp. and one as Cyanobium sp., which can be used as a support for the following genetical analyses.

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Authors and Affiliations

  1. Biological Center, Hydrobiological Institute of the Czech Academy of Sciences, České Budějovice, 37005, Czech Republic

    Jitka Jezberová

  2. Faculty of Biological Sciences, University of South Bohemia, České Budějovice, 37005, Czech Republic

    Jaroslava Komárková

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  1. Jitka Jezberová
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  2. Jaroslava Komárková
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Correspondence to Jitka Jezberová.

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Jezberová, J., Komárková, J. Morphometry and growth of three Synechococcus-like picoplanktic cyanobacteria at different culture conditions. Hydrobiologia 578, 17–27 (2007). https://doi.org/10.1007/s10750-006-0429-0

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