Abstract
Microbial eukaryote diversity was assessed in Arctic Kongsfjorden (Svalbard), by constructing SSU rDNA clone libraries. Samples were collected from different depths at the outer basin in summer (2006), plus an additional one glacial and one sediment sample. The libraries displayed diversity based on 284 full-length sequences. Four main phyla, namely, Alveolates, Stramenopiles, Cercozoans, and Metazoans were often screened in this fjord. Alveolate occupied the highest percentage of taxa in the library of surface sea water, besides the Metazoan-related clones. Moreover, dinoflagellates, diatoms, and pico-Prasinophytes were detected as prevalent phytoplankton through the analysis of libraries. Questions related to the quantity of these phytoplankton and their roles in the microbial food loop arose from an ecological viewpoint.
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Adl, S. M., A. G. B. Simpson, M. A. Farmer, R. A. Andersen, O. R. Anderson & J. R. Barta, 2005. The new higher level classification of eukaryotes with emphasis on the taxonomy of protist. Journal of Eukaryotic Microbiology 52: 399–451.
Agatha, S., M. C. Strüder-Kypke & A. Beran, 2004. Morphologic and genetic variability in the marine planktonic ciliate Laboea strobila Lohmann, 1908 (Ciliophora, Oligotrichia), with notes on its ontogenesis. Journal of Eukaryotic Microbiology 51: 267–281.
Alekseev, V., H. J. Dumont, J. Pensaert, D. Baribwegure & J. R. Vanfleteren, 2006. A redescription of Eucyclops serrulatus (Fischer, 1851) (Crustaceae: Copepoda: Cyclopoida) and some related taxa, with a phylogeny of the E. serrulatus-group. Zoologica Scripta 35: 123–147.
Buck, K. & D. Garrison, 1988. Distribution and abundance of choanoflagellates (Acanthoecidae) across the ice-edge zone in the Weddell Sea, Antarctica. Marine Biology 98: 263–269.
Chao, A., 1987. Estimating the population size for capture-recapture data with unequal catchability. Biometrics 43: 783–791.
Countway, P. D., R. J. Gast, P. Savai & D. A. Caron, 2005. Protistan diversity estimates based on 18S rDNA from seawater incubations in the Western North Atlantic. Journal Eukaryotic Microbiology 52: 95–106.
Díez, B., C. Pedrós-Alió & R. Masanna, 2001. Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rRNA gene cloning and sequencing. Applied and Environmental Microbiology 67: 2932–2941.
Edgcomb, V. P., D. T. Kysela, A. Teske, A. de Vera Gomez & M. L. Sogin, 2002. Benthic eukaryotic diversity in the Guaymas basin hydrothermal vent environment. Proceedings of the National Academy of Sciences United States of America 99: 7658–7662.
Edvardsen, B., W. Eikrem, J. C. Green, R. A. Andersen, S. Y. Moon-van der Staay & L. K. Medlin, 2000. Phylogenetic reconstructions of the Haptophyta inferred from 18S ribosomal DNA sequences and available morphological data. Phycologia 39: 19–35.
Fuller, N. J., C. Campbell, D. J. Allen, F. D. Pitt, K. Zwirglmaier, F. Le Gall, D. Vaulot & D. J. Scanlan, 2006. Analysis of photosynthetic picoeukaryote diversity at open ocean sites in the Arabian Sea using a PCR biased towards marine algal plastids. Aquatic Microbial Ecology 43: 79–93.
Gast, R. J., D. M. Moran, D. J. Beaudoin, J. N. Blythe, M. R. Dennett & D. A. Caron, 2006. Abundance of a novel dinoflagellate phylotype in the Ross Sea, Antarctica. Journal of Phycology 42: 233–242.
Gromov, B. V., A. V. Plujusch & K. A. Marnkaeva, 1999. Morphology and possible host range of Rhizophydium algavorum sp. nov. (Chytridiales)—an obligate parasite of algae. Protistology 1: 62–65.
Guillou, L., W. Eikrem, M. J. Chrétiennot-Dinet, F. Le Gall, R. Massana, K. Romari, C. Pedrós-Alió & D. Vaulot, 2004. Diversity of picoplanktonic prasinophytes assessed by direct nuclear SSU rDNA sequencing of environmental samples and novel isolates retrieved from oceanic and coastal marine ecosystems. Protist 155: 193–214.
Harada, A., S. Ohtsuka & T. Horiguchi, 2007. Species of the Parasitic Genus Duboscquella are members of the Enigmatic Marine Alveolate Group I. Protist 158: 337–347.
Hepperle, D., 2003. Align, Manual Sequence Alignment Editor for PCs. Distributed by the author. http://wwwuser.gwdg.de/~dhepper/.
Hop, H., T. Pearson, E. N. Hegseth, K. M. Kovacs, C. Wiencke, S. Kwasniewski, K. Eiane, F. Mehlum, B. Gulliksen, M. Wlodarska-Kowalezuk, C. Lydersen, J. M. Weslawski, S. Cochrane, G. W.Gabrielsen, R. J. G. Leakey, O. J. Lonne, M. Zajaczkowski, S. Falk-Petersen, M. Kendall, S. A. Wangberg, K. Bischof, A. Y. Voronkov, N. A. Kovaltchouk, J. Wiktor, M. Poltermann, G. di Prisco, C. Papucci & S. Gerland, 2002. The marine ecosystem of Kongsfjorden, Svalbard. Polar Research 21: 167–208.
Hughes, J. B., J. J. Hellmann, T. H. Ricketts & B. J. M. Bohannan, 2001. Counting the uncountable: statistical approaches to estimating microbial diversity. Applied and Environmental Microbiology 67: 4399–4406.
Ikävalko, J. & H. A. Thomsen, 1997. The Baltic Sea biota (March 1994): A study of the protistan community. European Journal of Protistology 33: 229–243.
Klaveness, D., K. Shalchian-Tibrizi, H. A. Thomsen, W. Eikrem & K. S. Jakobsen, 2005. Telonema antarcticum sp. nov., a common marine phagotrophic flagellate. International Journal of Systematic and Evolutionary Microbiology 55: 2595–2604.
Kühn, S., M. Lange & L. K. Medlin, 2000. Phylogenetic position of Cryothecomonas inferred from Nuclear-Encoded small subunit ribosomal RNA. Protist 151: 337–345.
Kwasniewski, S., H. Hop, S. Falk-Petersen & G. Pedersen, 2003. Distribution of Calanus species in Kongsfjorden, a glacial fjord in Svalbard. Journal of Plankton Research 25: 1–20.
Lefèvre, E., C. Bardot, C. Noēl, J. F. Carrias, E. Viscoglios, C. Amblard & T. Sime-Ngando, 2007. Unveiling fungal zooflagellates as members of freshwater picoeukaryotes: evidence from a molecular diversity study in a deep meromictic lake. Environmental Microbiology 9: 61–71.
López-García, P., F. Rodriguez-Valera & C. Pedrós-Alió, 2001. Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton. Nature 409: 603–606.
Lovejoy, C., R. Massana & C. Pedrós-Alió, 2006. Diversity and distribution of marine microbial eukaryotes in the Arctic Ocean and Adjacent seas. Applied and Environmental Microbiology 72: 3085–3095.
Lovejoy, C., W. F. Vincent, S. Bonilla & S. Roy, 2007. Distribution, phylogeny, and growth of cold-adapted picoprasinophytes in Arctic seas. Journal of Phycology 43: 78–89.
Luo, W., S. Pflugmacher, T. Pröschold, N. Walz & L. Krienitz, 2006. Genotype versus phenotype variability in Chlorella and Micractinium (Chlorophyta, Trebouxiophyceae). Protist 157: 315–333.
Massana, R., V. Balagué, L. Guillou & C. Pedrós-Alió, 2004. Picoeukaryotic diversity in an oligotrophic coastal site studied by molecular and culturing approaches. FEMS Microbiology Ecology 50: 231–243.
Medlin, L. K., H. J. Elwood, S. Stickel & M. L. Sogin, 1988. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71: 491–499.
Michaela, C., M. C. Strüder-Kypke & D. H. Lynn, 2003. Sequence analyses of the small subunit rRNA gene confirm the paraphyly of oligotrich ciliate sensu lato and support the monophyly of the subclasses Oligotrichia and Choreotrichia (Ciliophora, Spirotrichea). Journal of Zoology 260: 87–97.
Moon-van der Staay, S. Y., R. De Wachter & D. Vaulot, 2001. Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature 409: 607–610.
Not, F., M. Latasa & D. Marie, 2004. A single species Micromonas pusilla (Prasinophyceae), dominates the eukaryotic picoplankton in the Western English Channel. Applied Environmental Microbiology 70: 4064–4072.
Not, F., R. Masssana, M. Latasa, D. Marie, C. Colson, W. Eikrem, C. Pedrós-Alió, D. Vaulot & N. Simon, 2005. Late summer community composition and abundance of photosynthetic picoeukaryotes in Norwegian and Barents Seas. Limnology and Oceanography 50: 1677–1686.
Not, F., R. Gausling, F. Azam, J. F. Heidelberg & A. Z. Worden, 2007. Vertical distribution of picoeukaryotic diversity in the Sargasso Sea. Environmental Microbiology 9: 1233–1252.
Romari, K. & D. Vaulot, 2004. Composition and temporal variability of picoeukaryote communities at a coastal site of English Channel from 18S rDNA sequence. Limnology and Oceanography 49: 784–798.
Sarno, D., W. H. C. F. Kooistra, S. Balzano, P. E. Hargraves & A. Zingone, 2007. Diversity in the genus Skeletonema (Bacillariophyceae): III. Phylogenetic position and morphological variability of Skeletonema costatum and Skeletonema grevillei, with the description of Skeletonema ardens sp. nov. Journal of Phycology 43: 156–170.
Sime-Ngando, T., M. Gosselin, S. K. Juniper & M. Lavasseur, 1997. Changes in sea-ice phagotrophic microprotists (20–200 μm) during the spring algal bloom, Canadian Arctic Archipelago. Journal of Marine Systems 11: 163–172.
Stoeck, T. & S. Epstein, 2003. Novel eukaryotic lineages inferred from small-subunit rRNA analyses of oxygen-depleted marine environments. Applied Environmental Microbiology 69: 2657–2663.
Svendsen, H., A. Beszczynska-Moller, J. O. Hagen, B. Lefauconnier, V. Tverberg, S. Gerland, J. B. Orbaek, K. Bischof, C. Papucci, M. Zajaczkowski, R. Azzolini, O. Bruland, C. Wiencke, J. G. Winther & W. Dallmann, 2002. The physical environment of Kongsfjorden-Krossfjorden, an Arctic fjord system in Svalbard. Polar Research 21: 133–166.
Takishita, K., H. Miyake, M. Kawato & T. Maruyama, 2005. Genetic diversity of microbial eukaryotes in anoxic sediment around fumaroles on a submarine caldera floor based on the small-subunit rDNA phylogeny. Extremophiles 9: 185–196.
Thomsen, H., 1982. Planktonic choanoflagellates from Disko Bugt, West Greenland, with a survey of the marine nanoplankton of the area Meddelelser om Gronland. Bioscience 8: 3–36.
Throndsen, J. & S. Kristiansen, 1991. Micromonas pusilla (Prasinophyceae) as part of picoplankton and nanoplankton communities of the Barents Sea. Polar Research 10: 201–207.
Vaulot, D., F. L. Gall & D. Marie, 2004. The Roscoff culture collection (RCC): A collection dedicated to marine picoplankton. Nova Hedwigia 79: 49–70.
Vaulot, D., W. Eikrem, M. Viprey & H. Moreau, 2008. The diversity of small eukaryotic phytoplankton (<3 μm) in marine ecosystems. FEMS Microbiology Review 32: 795–820.
Wang, G. Z., C. Y. Guo, W. Luo, M. H. Cai & J. F. He, 2009. The distribution of picoplankton and nanoplankton in Kongsfjorden during late summer 2006. Polar Biology 32: 1233–1238.
Zeidner, G., C. M. Preston & E. F. Delong, 2003. Molecular diversity among marine picophytoplankton as revealed by psbA analyses. Environmental Microbiology 5: 212–216.
Acknowledgments
This research is supported by National Natural Science Foundation of China (No. 40806073, 40876097); the Youth Innovation foundation of Polar research Institute of China (JDQ200701); Polar Strategic Research Foundation of China; Youth Marine Science Foundation (2008128), SOA; and Resource-sharing Platform of Polar Samples (2005DKA21406). We acknowledge the logistic support from Kingsbay AS, Svalbard. We also appreciate the technical assistance from Q. Luo, YY. Lou, CJ. Zhu, HB. Teng, and CY. Guo. We also thank the reviewers for their advices.
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Luo, W., Li, H., Cai, M. et al. Diversity of microbial eukaryotes in Kongsfjorden, Svalbard. Hydrobiologia 636, 233–248 (2009). https://doi.org/10.1007/s10750-009-9953-z
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DOI: https://doi.org/10.1007/s10750-009-9953-z