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Role of NLRP3 Inflammasome in Eosinophilic and Non-eosinophilic Chronic Rhinosinusitis with Nasal Polyps

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Abstract

The pathophysiologic mechanisms of human chronic rhinosinusitis with nasal polyps (CRSwNP) remain unclear. We aimed to elucidate expression and biologic role of NLRP3 inflammasome in CRSwNP. Immunohistochemistry (IHC) was conducted to assess NLRP3 immunolabeling, real-time polymerase chain reaction (PCR) was used for IL-9 and NLRP3, and caspase-1 level quantitation in CRSwNP and control subjects. In addition, enzyme-linked immunosorbent assay (ELISA) was employed for analyzing concentrations of IL-1β and IL-18 in the homogenates prepared from tissue specimens. Moreover, human nasal epithelial cells (HNECs) were used to evaluate the effects of lipopolysaccharide (LPS) and glyburide on NLRP3 inflammasome signaling pathway. Results showed that NLRP3 and caspase-1 were overexpressed in CRSwNP, especially in eosinophilic CRSwNP (ECRSwNP). Interestingly, NLRP3 expression had close correlation to that of caspase-1. Concentrations of IL-1β and IL-18 were elevated. NLRP3 inflammasome signaling pathway was augmented by LPS but suppressed by glyburide. In conclusion, NLRP3 inflammasome signaling pathway played a pro-inflammatory role in the pathogenesis of CRSwNP, especially in ECRSwNP. NLRP3 inflammasome signaling pathway was augmented by LPS, but suppressed by glyburide.

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Acknowledgments

This work was supported by Fujian Provincial Natural Science Foundation of China (No. 2010 J01215 and No. 2015 J01302).

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    Authors and Affiliations

    1. Department of Otorhinolaryngology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, 200233, China

      Hai Lin, Zhipeng Li & Weitian Zhang

    2. Department of Otorhinolaryngology, Fuzhou General Hospital, Fuzhou, 350025, Fujian, China

      Hai Lin

    3. Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, Fuqing, 350300, Fujian, China

      Dong Lin

    4. Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, 200031, China

      Chunquan Zheng

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    1. Hai Lin
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    2. Zhipeng Li
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    3. Dong Lin
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    5. Weitian Zhang
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    Correspondence to Hai Lin or Weitian Zhang.

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    This study was approved by the ethical committees of Shanghai Jiao Tong University Affiliated Sixth People’s Hospital and Fuzhou General Hospital, and an informed consent was provided from every subject.

    Additional information

    Hai Lin, Zhipeng Li and Dong Lin contributed equally to this work.

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    Lin, H., Li, Z., Lin, D. et al. Role of NLRP3 Inflammasome in Eosinophilic and Non-eosinophilic Chronic Rhinosinusitis with Nasal Polyps. Inflammation 39, 2045–2052 (2016). https://doi.org/10.1007/s10753-016-0442-z

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    • DOI: https://doi.org/10.1007/s10753-016-0442-z

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