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Effects of Ergosterol on COPD in Mice via JAK3/STAT3/NF-κB Pathway

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Abstract

The present study was to evaluate the effect of ergosterol (ER) on CS (cigarette smoke)-induced chronic obstructive pulmonary disease (COPD) in mice. Fifty male ICR mice were randomly assigned to five groups: control group, CS group, CS + dexamethasone (Dex, 2 mg/kg) group, CS + ER (ER, 25 mg/kg) group, CS + ER (ER, 50 mg/kg). H&E staining demonstrated that ER inhibited CS-induced pathological injury in lung tissue. Besides, ER could restore the activities of superoxide dismutase (SOD) in serum and in the lung, catalase (CAT) in serum and reduce the content of malondialdehyde (MDA) in serum and in the lung. ER also inhibited pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in serum and the lung. Furthermore, ER significantly inhibited the protein expression of JAK3/STAT3/NF-κB pathway in CS-induced mice. Our findings suggested that ER might effectively ameliorate the progression of COPD via JAK3/STAT3/NF-κB pathway in mice.

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Acknowledgements

This work was supported by the National Twelve Five Major Drug Discovery Project (2011ZX09102-002-01). This research was supported by National Natural Science Foundation of China (No. 81274037).

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Authors and Affiliations

  1. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University Jilin, Changchun, 130118, China

    Wang Huan, Zhang Tianzhu & Li Yu

  2. Changchun University of Chinese Medicine, Changchun, Jilin, 130117, China

    Wang Shumin

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Correspondence to Li Yu or Wang Shumin.

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Huan, W., Tianzhu, Z., Yu, L. et al. Effects of Ergosterol on COPD in Mice via JAK3/STAT3/NF-κB Pathway. Inflammation 40, 884–893 (2017). https://doi.org/10.1007/s10753-017-0533-5

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  • DOI: https://doi.org/10.1007/s10753-017-0533-5

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