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Arctiin Prevents LPS-Induced Acute Lung Injury via Inhibition of PI3K/AKT Signaling Pathway in Mice

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Abstract

Arctiin is a lignin isolated from Arctium lappa which has been known to have anti-viral and anti-inflammatory effects. The aim of this study is to explore the protective effect of arctiin on lipopolysaccharide (LPS)-induced inflammatory responses in acute lung injury (ALI) model of mice. Male BALB/c mice were pretreated with commercial arctiin (10, 20, and 40 mg/kg) 1 h prior to LPS challenge. Twelve hours later, airway inflammation was assessed. We assessed the effects of arctiin on the LPS-induced production of TNF-α, IL-6, and IL-1β in the bronchoalveolar lavage fluid (BALF). The lung wet-to-dry weight ratio, myeloperoxidase (MPO) activity, and inflammatory signaling pathway were also detected. Our results showed that arctiin not only significantly ameliorated LPS-stimulated lung histopathological changes but also reduced the lung MPO activity. Arctiin also dramatically decreased the production of TNF-α, IL-1β, and IL-6 in the BALF. In addition, arctiin significantly inhibited LPS-induced PI3K/Akt phosphorylation as well as NF-κB activation. In conclusion, our results suggested that arctiin protected against LPS-induced ALI through inhibiting PI3K/AKT/NF-κB signaling pathway.

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Author notes

  1. Bo Zhou and Guohu Weng are the co-first author.

Authors and Affiliations

  1. Department of Intensive Care Unit, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China

    Bo Zhou & Feiyue Dai

  2. Department of Neurology, Hainan Provincial Hospital of TCM, Haikou, Hainan, China

    Guohu Weng & Tao Liu

  3. Department of Cardiology, Hainan Provincial Hospital of TCM, Haikou, Hainan, China

    Zhengxin Huang

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  1. Bo Zhou
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  2. Guohu Weng
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  3. Zhengxin Huang
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  4. Tao Liu
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  5. Feiyue Dai
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Contributions

B.Z. and F.D. contributed to the conception and design. B. Z., G.W., Z.H., and T.L. did the analysis and interpretation. T.L. did the data collection. B.Z. and F.D. wrote the article and did the critical revision of the article.

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Correspondence to Feiyue Dai.

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The authors declare that they have no conflict of interest.

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Zhou, B., Weng, G., Huang, Z. et al. Arctiin Prevents LPS-Induced Acute Lung Injury via Inhibition of PI3K/AKT Signaling Pathway in Mice. Inflammation 41, 2129–2135 (2018). https://doi.org/10.1007/s10753-018-0856-x

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