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Protective Effects of Sweroside on IL-1β-Induced Inflammation in Rat Articular Chondrocytes Through Suppression of NF-κB and mTORC1 Signaling Pathway

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Abstract

Sweroside (SW), as a bioactive herbal ingredient, has anti-inflammatory effects. Protective effects of SW on IL-1β-stimulated articular chondrocytes, however, has not been fully understood. This study was to explore the anti-inflammatory effects and further to investigate the possible mechanism underlying SW effect on IL-1β-stimulated rat articular chondrocytes. Rat articular chondrocytes were cultured with or without SW for 1 h, and then stimulated with IL-1β for 24 h. ELISA analysis was used to measure the production of NO and PGE2. Western blot was to detect the expression of iNOS and COX-2. Furthermore, the mRNA expression of MMP-1, MMP3, MMP13, and ADAMTS-5 were measured by q-PCR. These results demonstrated that SW significantly inhibited IL-1β-induced NO and PGE2 production, as well as MMP-1, MMP3, MMP13, and ADAMTS-5 mRNA expression. Moreover, SW also suppressed IL-1β-induced NF-κB activation and iκ-B degradation, S6K1 and S6 phosphorylation. In conclusion, these results strongly demonstrated that the anti-inflammatory activity of SW is in part mediated by suppressing NF-κB and mTORC1 signaling, which was expected to be a promising drug target of osteoarthritis therapy.

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Author notes

  1. Rui Zhang, Chao-min Wang and Hua-ji Jiang contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiology (Cardiac Rehabilitation), The Third Affiliated Hospital of Southern Medical University, Tianhe District, Guangzhou, China

    Rui Zhang

  2. Department of Trauma Treatment Center, The Third Affiliated Hospital of Southern Medical University, Tianhe District, Guangzhou, China

    Chao-min Wang

  3. Department of Pain, Yue Bei People’s Hospital, No. 133 South Huimin Road,, Shaoguan, Guangdong, China

    Hua-ji Jiang, WenJun Li, Wei Liang, Jianhua Yang & Chunlan Zhong

  4. Department of Orthopedics, The Affiliated Hospital of Southwestern Medical University, LuZhou, China

    Xing-gui Tian

  5. Department of Orthopedics, The Third Affiliated Hospital of Southern Medical University, No. 183 West Zhongshan Road, Tianhe District, Guangzhou, 510630, China

    Yuhui Chen & Tao Li

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  1. Rui Zhang
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Correspondence to Yuhui Chen or Tao Li.

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Zhang, R., Wang, Cm., Jiang, Hj. et al. Protective Effects of Sweroside on IL-1β-Induced Inflammation in Rat Articular Chondrocytes Through Suppression of NF-κB and mTORC1 Signaling Pathway. Inflammation 42, 496–505 (2019). https://doi.org/10.1007/s10753-018-0906-4

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