Abstract
Bengal slow lorises (Nycticebus bengalensis) and pygmy slow lorises (Nycticebus pygmaeus) are nocturnal which creates difficulties to study them in the field. There is a scarcity of data on them and their population genetics are poorly understood. We sequenced and analyzed a partial fragment in the first hypervariable region of the mitochondrial control region or D-loop HVRI of 21 Nycticebus bengalensis and 119 N. pygmaeus from the boundary between China and Vietnam where they are sympatric. Though the sample size for Nycticebus pygmaeus is much larger, the polymorphism level is much lower than that of N. bengalensis, possibly because of (1) external gene flow from other habitats of N. bengalensis, (2) gene ingression from Sunda slow lorises (N. coucang coucang) to N. bengalensis, (3) a skewed birth sex ratio in N. pygmaeus, and (4) a possible low survival rate of infant N. pygmaeus. Based on genetic comparisons to Nycticebus bengalensis, we propose that N. pygmaeus in southern China and northern Vietnam might have migrated from middle or southern Vietnam recently.
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References
Avise, J. C. (1991). Ten unorthodox perspectives on evolution prompted by comparative population genetic findings on mitochondrial DNA. Annual Review of Genetics, 25, 45–69.
Bandelt, H. J., Forster, P., Sykes, B. C., and Richards, M. B. (1995). Mitochondrial portraits of human populations using median networks. Genetics, 141, 743–753.
Chen, J. H., Pan, D., Groves, C., Wang, Y. X., Narushima, E., Fitch-Snyder, H., et al. (2005). Molecular phylogeny of Nycticebus inferred from mitochondrial genes. International Journal of Primatolology, In press.
Easteal, S. (1991). The relative rate of DNA evolution in primates. Molecular Biology and Evolution, 8, 115–127.
Excoffier, L., and Yang, Z. (1999). Substitution rate variation among sites in mitochondrial hypervariable region of humans and chimpanzees. Molecular Biology and Evolution, 16, 1357–1368.
Fitch-Snyder, H. (2000). Reproductive patterns in a breeding colony of pygmy lorises (Nycticebus pygmaeus) Measured by behavioral and physiological correlates of gonadal activity. San Diego: San Diego State University.
Griffiths, R. C., and Tavare, S. (1994). Sampling theory for neutral alleles in a varying environment. Philosophical Transactions of the Royal Society of London Series. B-Biological Science, 344, 403–410.
Groves, C. P. (2001). Primate taxonomy. Washington, D.C: Smithsonian Institution Press.
Jazin, E., Soodyall, H., Jalonen, P., Lindholm, E., Stoneking, M., and Gyllenstein, U. (1998). Mitochondrial mutation rate revisited: Hot spots and polymorphism. Nature Genetics, 18, 109–110.
Kumar, S., Tamura, K., Jakobsen, I. B., and Nei, M. (2001). MEGA2: molecular evolutionary genetics analysis software. Bioinformatics, 17, 1244–1245.
Li, W. H., and Graur, D. (1991). Fundamentals of molecular evolution. Sunderland, MA: Sinauer Associates.
Lu, X. M., Fu, Y. X., and Zhang, Y. P. (2002). Evolution of mitochondrial cytochrome b pseudogene in genus Nycticebus. Molecular Biology and Evolution, 19, 2337–2341.
Lu, X. M., Wang, Y. X., and Zhang, Y. P. (2001). Divergence and phylogeny of mitochondrial cytochrome b gene from species in genus Nycticebus. Zoology Research, 22, 93–98.
Nei, M. (1987). Molecular evolutionary genetics. New York: Columbia University Press.
Oxnard, C. E., Crompton, R. H., and Lieberman, S. S. (1990). The case of the prosimian primates. Seattle: University of Washington Press.
Parsons, T. J., and Holland, M. M. (1998). Reply to Jazin et al. Nature Genetics, 18, 110.
Parsons, T. J., Muniec, D. S., Sullivan, K., Woodyatt, N., Alliston-Greiner, R., Wilson, M. R., et al. (1997). A high observed substitution rate in the human mitochondrial DNA control region. Nature Genetics, 15, 363–368.
Raaum, R. L., Sterner, K. N., Noviello, C. M., Stewart, C. B., and Disotell, T. R. (2005). Catarrhine primate divergence dates estimated from complete mitochondrial genomes: Concordance with fossil and nuclear DNA evidence. Journal of Human Evolution, 48, 237–257.
Rozas, J., Sanchez-Delbarrio, X. M., and Rozas, R. (2003). DNASP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19, 2496–2497.
Tajima, F. (1983). Evolutionary relationship of DNA sequences in finite populations. Genetics, 105, 437–460.
Tajima, F. (1989). Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, 123, 585–595.
Tan, C. L., and Drake, J. H. (2001). Evidence of tree gouging and exudate eating in pygmy slow lorises (Nycticebus pygmaeus). Folia Primatologica, 72, 37–39.
Wang, S. (1998). China red data book of endangered animals. Beijing, Hong Kong, New York: Science Press.
Watterson, G. A. (1975). On the number of segregating sites in genetical models without recombination. Theoretical Population Biology, 7, 256–276.
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The Bureau of Science and Technology of Yunnan Province, and National Natural Science Foundation of China (grants 30460026, 30621092) provided funding for this work.
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Deng Pan and Jing-Hua Chen made equal contributions to the work.
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Pan, D., Chen, JH., Groves, C. et al. Mitochondrial Control Region and Population Genetic Patterns of Nycticebus bengalensis and N. pygmaeus . Int J Primatol 28, 791–799 (2007). https://doi.org/10.1007/s10764-007-9157-1
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DOI: https://doi.org/10.1007/s10764-007-9157-1