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Bromfenac ophthalmic solution 0.09 %: human aqueous humor concentration detected by high-performance liquid chromatography

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Abstract

The purpose of this study was to evaluate the aqueous humor concentrations of bromfenac ophthalmic solution 0.09 % in patients undergoing phacoemulsification. Patients requiring cataract extraction received one drop (50 µL) of bromfenac 0.09 % solution in the eye to be operated, before bedtime the day before surgery or the morning of the surgery. The last administration was recorded. At the time of paracentesis, an aqueous humor sample was collected with a 30-gauge needle attached to a TB syringe and was later analyzed by high-performance liquid chromatography for drug concentration. 188 treated volunteers and 48 control, untreated, subjects were included in the study. The mean aqueous concentration of bromfenac in the treated group was 37.60 ± 68.86 and 0 nM (nmol/L) in the control group (p < 0.0001). Correlation coefficient in bromfenac group between time elapsed from instillation and drug concentration was −0.16 (p not significant). Bromfenac showed properties of good penetration and stable concentration in aqueous humor up to about 12 h after instillation.

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Authors and Affiliations

  1. Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genoa, Via San Luca d’Albaro 37/1, 16146, Genoa, Italy

    Angelo Macrì, Aldo Vagge & Michele Iester

  2. Center of Excellence for Biomedical Research (CEBR), University of Genoa, Viale Benedetto XV 5, 16132, Genoa, Italy

    Annalisa Salis & Gianluca Damonte

  3. Department of Internal Medicine, Pharmacology and Toxicology Unit, University of Genoa, Genoa, Italy

    Carmen Fucile, Valeria Marini, Antonietta Martelli & Francesca Mattioli

  4. Bausch + Lomb Iom SpA, Viale Martesana 12, 20090, Vimodrone, MI, Italy

    Sebastiano Giuffrida

  5. Department of Experimental Medicine, Section of Biochemistry, University of Genoa, Viale Benedetto XV 1, 16132, Genoa, Italy

    Gianluca Damonte

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  1. Angelo Macrì
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  2. Aldo Vagge
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  3. Annalisa Salis
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  4. Carmen Fucile
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Correspondence to Angelo Macrì.

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Macrì, A., Vagge, A., Salis, A. et al. Bromfenac ophthalmic solution 0.09 %: human aqueous humor concentration detected by high-performance liquid chromatography. Int Ophthalmol 37, 417–421 (2017). https://doi.org/10.1007/s10792-016-0279-3

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  • DOI: https://doi.org/10.1007/s10792-016-0279-3

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