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Preliminary Experience with Low Concentration of Granulocyte-Macrophage Colony-Stimulating Factor: A Potential Regulator in Preimplantation Mouse Embryo Development and Apoptosis

  • Animal Experimentation
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Abstract

Purpose: To investigate the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the development of preimplantation mouse embryos.

Methods: Mouse 2-cell embryos were collected and cultured in P-1 medium supplemented with GM-CSF at different concentrations. Using reverse transcription-polymerase chain reaction, expression Bcl-2 and Bax mRNA in blastocyst were evaluated in the GM-CSF group and control group. Apoptosis detection was performed using the in situ apoptosis detection kit in mouse blastocysts. The statistical significance of the data was analyzed using t-test and chi-square test.

Results: The development of blastocyst increased to 89% in the addition of GM-CSF (0.125 ng/mL), compared to controlled group (80%). The number of cells staining for apoptosis was lower in GM-CSF group than that in the control group. Bcl-2 expression was found to be upregulated in blastocysts in the GM-CSF supplemented group compared to the control group.

Conclusion: These results suggest that GM-CSF might be an important regulator in embryo development.

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Authors and Affiliations

  1. Stanford University School of Medicine, Stanford, California

    Barry Behr, Stephen Mooney, Yan Wen, Mary Lake Polan & Hongbo Wang

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  1. Barry Behr
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  2. Stephen Mooney
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  3. Yan Wen
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  4. Mary Lake Polan
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  5. Hongbo Wang
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Correspondence to Barry Behr.

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Behr, B., Mooney, S., Wen, Y. et al. Preliminary Experience with Low Concentration of Granulocyte-Macrophage Colony-Stimulating Factor: A Potential Regulator in Preimplantation Mouse Embryo Development and Apoptosis. J Assist Reprod Genet 22, 25–32 (2005). https://doi.org/10.1007/s10815-005-0817-9

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  • DOI: https://doi.org/10.1007/s10815-005-0817-9

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