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Effect of the glutaraldehyde derivatives of Calix[n]arene as cross-linker reagents on lipase immobilization

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Abstract

Synthesis of the glutaraldehyde derivatives calix[n]arene (n = 4,6,8) (Calix[n]-GA) and using as cross-linkers for immobilization of Candida rugosa lipase (CRL) have been discussed in this paper. The amino functional calix[n]arene derivatives (Calix[n]-NH 2) were synthesized via reduction of dinitrile, hexanitrile and octanitrile derivatives of calix[n]arenes. These amino functional calix[n]arene derivatives (Calix[n]-NH 2) were converted to their aldehyde derivativatives with glutaraldehyde. The calix[n]arene derivatives were used in lipase immobilization in order to see the role of calix[n]arene binding site on the lipase activitiy and stability. The activity recovery of calix[n]arene-supported lipases (Calix[n]-CRL) based on the Calix[4]-CRL, Calix[6]-CRL and Calix[8]-CRL reaches to 53.5, 66.1 and 76.4%, respectively.

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Acknowledgments

We would like to thank The Research Foundation of Selcuk University (BAP) and The Scientific and Technical Research Council of Turkey (TUBITAK-Grant Number 106T435) for financial support of this work.

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  1. Department of Chemistry, Selcuk University, 42031, Konya, Turkey

    Serkan Erdemir, Ozlem Sahin, Arzu Uyanik & Mustafa Yilmaz

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  1. Serkan Erdemir
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Correspondence to Mustafa Yilmaz.

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Erdemir, S., Sahin, O., Uyanik, A. et al. Effect of the glutaraldehyde derivatives of Calix[n]arene as cross-linker reagents on lipase immobilization. J Incl Phenom Macrocycl Chem 64, 273–282 (2009). https://doi.org/10.1007/s10847-009-9562-5

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