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Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite

  • In Honor of Larry Hench
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Abstract

Silicate ions released from bioactive glasses and ceramics have been reported to stimulate osteogenic cell functions. Here, we evaluated osteoblast-like cell reactions to silicate ions released from two different types of materials, 45S5 bioactive glass (BG) and siloxane-doped vaterite (SiV), to investigate the influence of the ionic structure of silicate ions on osteoblast-like cell properties. BG and SiV powders were prepared by using melt-quenching and carbonation methods, respectively. Aminopropyltriethoxysilane was used as a siloxane source of SiV. MC3T3-E1 and SaOS-2 cells were cultured in media containing dissolved BG or SiV ions (10–50 ppm of Si). Cell proliferation (metabolic activity), differentiation (alkaline phosphatase activity) and mineralisation (Ca deposition) were examined. 29Si NMR spectra demonstrated that Q0,1 species and T0–3 species were released from BG and SiV, respectively. Proliferation and mineralisation of the two types of cells were influenced by silicate ions released from BG and SiV in a concentration-dependent manner. In particular, there were significant differences (P < 0.05) in the degree of proliferation and Ca deposition levels in SaOS-2 cells treated with dissolved BG and SiV ions. Furthermore, Ca deposition in SaOS-2 cells was influenced by both the presence of silicate ions and the duration of exposure of cells to them. The structure of silicate ions influenced the proliferation and mineralisation of SaOS-2 cells incubated for different time periods in culture media containing different Si concentrations. Understanding the effect of Si on bone cell behaviour will enable a design-led approach to further BG optimisation.

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Acknowledgements

This work was supported in part by JSPS KAKENHI Grant Number 26820304 and Izumi Science and Technology Foundation.

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Authors and Affiliations

  1. Division of Advanced Ceramics, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, 466-8555, Japan

    Akiko Obata, Norihiko Iwanaga, Arisa Terada & Toshihiro Kasuga

  2. Division of Surgery and Interventional Science, Royal Free NHS Trust Hospital, University College London, 9th Floor, Pond Street, London, NW3 2QG, UK

    Gavin Jell

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  1. Akiko Obata
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  2. Norihiko Iwanaga
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  3. Arisa Terada
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Correspondence to Akiko Obata.

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Obata, A., Iwanaga, N., Terada, A. et al. Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite. J Mater Sci 52, 8942–8956 (2017). https://doi.org/10.1007/s10853-017-1057-y

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