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Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds

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Abstract

Insulin like growth factor 1 receptor (IGF-1R) is a candidate gene for growth and carcass traits in regulating animal growth, metabolism and endocrine. It is widely expressed in liver, muscle, bone tissues where the IGF-1R functions as a factor that promotes cell growth. In this study, the protein expression level of IGF-1R gene in liver and muscle tissues of three periods (birth, weaning and adult) of three pig breeds (BamaXiang pigs (BM), Tibetan pigs (TM) and Junmu No.1 pigs (JM)) were tested by western blot. SNPs within the regulatory region of pig IGF-1R gene were detected using direct sequencing and then the genotypes were identified through AS-PCR approach. Results showed expression profiles of IGF-1R gene between liver and muscle tissues were different and significant differences were also found among pig breeds. In the same time, four SNPs were detected in the regulatory region of IGF-1R gene, among which the genotype frequency of three (g.−1468G > C, g.−1192 C > T and g.330,424 C > T) were significantly different among the pig breeds. BM tended to heterozygous (GC/CT) of the anterior two loci, while TM and JM preferred the other two homozygotes respectively. For the g.330,424 C > T, all pig breeds were tended to be the heterozygous. In conclusion, the SNPs with different genotype distribution among the three pig breeds may explain the gene expression difference between the different pig breeds.

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Abbreviations

IGF-1R:

Insulin like growth factor 1 receptor

AS-PCR:

Allele-specific-PCR

References

  1. Baker J, Liu JP, Robertson EJ, Efstratiadis A (1993) Role of insulin-like growth factors in embryonic and postnatal growth. Cell 75:73–82

    Article  CAS  Google Scholar 

  2. Denley A, Cosgrove LJ, Booker GW, Wallace JC, Forbes BE (2005) Molecular interactions of the IGF system. Cytokine Growth Factor Rev 16:421–439

    Article  CAS  Google Scholar 

  3. Dricu A, Kanter L, Wang M, Nilsson G, HjerTPan M, Wejde J, Larsson O (1999) Expression of the insulin-like growth factor 1 receptor (IGF1R) in breast cancer cells: evidence for a regulatory role of dolichyl phosphate in the transition from an intracellular to an extracellular IGF1 pathway. Glycobiology 9:571–579

    Article  CAS  Google Scholar 

  4. Leroith D, Werner H, Beitner-Johnson D, Roberts CT (1995) Molecular and cellular aspects of the insulin-like growth factor I receptor. Endocr Rev 16:143–163

    Article  CAS  Google Scholar 

  5. Kawashima Y, Kanzaki S, Yang F, Kinoshita T, Hanaki K, Nagaishi J, Ohtsuka Y, Hisatome I, Ninomoya H, Nanba E, Fukushima T, Takahashi S (2005) Mutation at cleavage site of insulin-like growth factor receptor in a short-stature child born with intrauterine growth retardation. J Clin Endocrinol Metab 90:4679–4687

    Article  CAS  Google Scholar 

  6. Lei M, Peng X, Zhou M, Luo C, Nie Q, Zhang X (2008) Polymorphisms of the IGF1R gene and their genetic effects on chicken early growth and carcass traits. BMC Genet 9:70

    Article  Google Scholar 

  7. Moe HH, Shimogiri T, Kamihiraguma W, Isobe H, Kawabe K, Okamoto S, Minvielle F, Maeda Y (2007) Analysis of polymorphisms in the insulin-like growth factor 1 receptor (IGF1R) gene from Japanese quail selected for body weight. Anim Genet 38:659–661

    Article  CAS  Google Scholar 

  8. Hoopes BC, Rimbault M, Liebers D, Ostrander EA, Sutter NB (2012) The insulin-like growth factor 1 receptor (IGF1R) contributes to reduced size in dogs. Mamm Genome 23:780–790

    Article  CAS  Google Scholar 

  9. Terman A (2011) The IGF1R gene: a new marker for reproductive performance traits in sows? Acta Agric Scand A 61:67–71

    CAS  Google Scholar 

  10. Abuzzahab MJ, Schneider A, Goddard A, Grigorescu F, Lautier C, Keller E, Kiess W, Klammt J, Kratzsch J, Osgood D, Pfaffle R, Raile K, Seidel B, Smith RJ, Chernausek SD (2003) IGF-I receptor mutations resulting in intrauterine and postnatal growth retardation. New Eng J Med 349:2211–2222

    Article  CAS  Google Scholar 

  11. Li Zicong, Zhenfang Wu, Ren Guangcai, Zhao Yunxiang, Liu Dewu (2013) Expression patterns of insulin-like growth factor system members and their correlations with growth and carcass traits in Landrace and Lantang pigs during postnatal development. Mol Biol Rep 40:3569–3576

    Article  CAS  Google Scholar 

  12. Wu FC, Wei H, Gan SX, Wang AD (2001) Analysis of genetic diversity in Bama and Guizhou miniature pigs by RAPD. Shi Yan Sheng Wu Xue Bao 34:115–119 (in Chinese)

    CAS  Google Scholar 

  13. Liu HB, Lv PR, He RG, Yang XG, Qin XE, Pan TB, Huang GY, Huang MR, Lu YQ, Lu SS, Li DS, Lu KH (2010) Cloned Guangxi Bama minipig (Sus scrofa) and its offspring have normal reproductive performance. Cell Reprogram 12:543–550

    Article  CAS  Google Scholar 

  14. Li M, Tian S, Jin L, Zhou G, Li Y (2013) Genomic analyses identify distinct patterns of selection in domesticated pigs and Tibetan wild boars. Nat Genet 45(12):1431–1438

    Article  CAS  Google Scholar 

  15. Li JY, Luo Z (1993) A research on the habits and characteristics of Tibet pigs on Tibet plateau. Ecol Domest Anim 14(1):18–21

    Google Scholar 

  16. XU Xiaoyan, XIA Pu, XING Yanan, Guan Yifu, Zheng Huachuan (2010) Tissue protein extraction by freezing and breaking method. J China Med Univ 39(5):336–339 (in Chinese)

    CAS  Google Scholar 

  17. Pang WJ, Wang Y, Wei N, Xu RX, Xiong Y, Wang P, Shen QW, Yang GS (2013) Sirt1 inhibits akt2-mediated porcine adipogenesis potentially by direct protein-protein interaction. PLoS One 8(8):e71576

    Article  CAS  Google Scholar 

  18. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad 70:3321–3323

    Article  CAS  Google Scholar 

  19. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Human Genet 32:314–331

    CAS  Google Scholar 

  20. National Committee for Livestock or Poultry Genetic Resource (2011) Chinese livestock or poultry genetic resource-pigs. China Agriculture Press, pp 237, 268, 427, 454–454 (in Chinese)

  21. Delafontaine P, Song YH, Li Y (2004) Expression, regulation, and function of IGF1, IGF1R, and IGF1 binding proteins in blood vessels. Arterioscler Thromb Vasc Biol 24:435–444

    Article  CAS  Google Scholar 

  22. Charge SB, Rudnicki MA (2004) Cellular and molecular regulation of muscle regeneration. Physiol Rev 84:209–238

    Article  CAS  Google Scholar 

  23. Tomas FM, Knowles SE, Owens PC, Burgoyne JL, Chandler CS, Ballard FJ (1996) Conjoint IGF1 and insulin infusion shows diverse interactive effects in diabetic rats. Diabetes 45:170–177

    Article  CAS  Google Scholar 

  24. Wyszynska-Koko J, Pierzchala M, Flisikowski K, Kamyczek M, Rozycki M, Kuryl J (2006) Polymorphisms in coding and regulatory regions of the porcine MYF6 and MYOG genes and expression of the MYF6 gene in m. Longissimus dorsi versus productive traits in pigs. J Appl Genet 47:131–138

    Article  Google Scholar 

  25. Bonafe M, Barbieri M, Marchegiani F, Olivieri F, Ragno E, Giampieri C, Mugianesi E, Centurelli M, Franceschi C, Paolisso G (2003) Polymorphic variants of insulin-like growth factor1 (IGF1) receptor and phosphoinositide 3-kinase genes affect IGF1 plasma levels and human longevity: cues for an evolutionarily conserved mechanism of life span control. J Clin Endocrinol Metab 88:3299–3304

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31101781, 31072102) and the Programs Foundation of Ministry of Education of China (20110061110081).

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    Authors and Affiliations

    1. College of Animal Science, Jilin University, 5333 Xi’an Road, Changchun, Jilin, 130062, China

      Yunyun Cheng, Songcai Liu, Qingyan Wu, Siming Li, Haoyu Fu, Lijie Dong, Hao Yu & Linlin Hao

    2. Five-Star Animal Health Pharmaceutical Factory of Jilin Province, 5333 Xi’an Road, Changchun, Jilin, 130062, China

      Songcai Liu

    3. China-Japan Union Hospital, Jilin University, 5333 Xi’an Road, Changchun, Jilin, 130062, China

      Xin Zhang

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    1. Yunyun Cheng
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    2. Songcai Liu
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    3. Xin Zhang
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    4. Qingyan Wu
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    5. Siming Li
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    9. Linlin Hao
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    Correspondence to Linlin Hao.

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    Yunyun Cheng and Songcai Liu contributed equally to this work.

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    Cheng, Y., Liu, S., Zhang, X. et al. Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds. Protein J 35, 231–236 (2016). https://doi.org/10.1007/s10930-016-9666-x

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