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Biological affinity evaluation of Lawsonia inermis origin Lawsone compound and its radioiodinated form via in vitro methods

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Abstract

WST-1-based cytotoxicity assay of lawsone (LW) was performed on MCF7, Caco2, BJ and Keratinocyte cells and viabilities were found as over 90 % for all cells. Significant wound healing effect of LW was reported on Keratinocyte cells. Antibacterial and antifungal activities of LW were tested on seven microorganisms with three concentrations and 1,000 µg/disc of LW showed antibacterial effect on Bacillus subtilis. In vitro cell incorporation of radioiodinated LW (131I-LW) was evaluated on same cells. Keratinocyte cells uptake were 5 times more. Consequently, 131I-LW was found usable for researches about especially skin diseases in addition to breast and intestinal cancer.

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Acknowledgments

This study has been supported by Ege University Research Fund (contract no 2013 NBE 004). The authors thank Prof. Serhan Sakarya for his theoretical support.

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Authors and Affiliations

  1. Department of Nuclear Applications, Institute of Nuclear Science, Ege University, 35100, Bornova, Izmir, Turkey

    Volkan Tekin, F. Zumrut Biber Muftuler, Ozge Kozgus Guldu, Ayfer Yurt Kilcar, E. Ilker Medine & Perihan Unak

  2. Department of Chemistry, Faculty of Science, Dicle University, 21280, Diyarbakir, Turkey

    Murat Yavuz

  3. Department of Biochemistry, Faculty of Sciences, Ege University, 35100, Bornova, Izmir, Turkey

    Murat Yavuz & Suna Timur

Authors
  1. Volkan Tekin
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  2. F. Zumrut Biber Muftuler
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Correspondence to F. Zumrut Biber Muftuler.

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Tekin, V., Biber Muftuler, F.Z., Kozgus Guldu, O. et al. Biological affinity evaluation of Lawsonia inermis origin Lawsone compound and its radioiodinated form via in vitro methods. J Radioanal Nucl Chem 303, 701–708 (2015). https://doi.org/10.1007/s10967-014-3435-0

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  • DOI: https://doi.org/10.1007/s10967-014-3435-0

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