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Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia

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Abstract

The changes in the regulation of at mitochondrial NADP-isocitrate dehydrogenase (NADP-ICDH) in a rat heart during have been analysed. Increase of enzyme activity in the cytosol and mitochondria of the heart ischemia was detected. Catalytic properties of the mitochondrial NADP-ICDH at norm and pathology have been compared on homogeneous enzyme preparations. Enzyme from the normoxic and ischemic heart showed the same electrophoretical mobility and molecular mass. Enzyme isolated from the ischemic heart mitochondria demonstrated higher activation energy and lower thermal stability. NADP-isocitrate dehydrogenase at the normoxic and ischemic conditions exhibited different Km for substrates and regulatory behaviour in relation to ATP, ADP, 2-oxoglutarate, citrate, malate, reduced and oxidised glutathione. The inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals was lower in the ischemic enzyme. We hypothesise that the specific features of regulation behaviour of NADP-ICDH from the ischemic tissues permits the enzyme to supply NADPH to the glutathione reductase/glutathione peroxidase system.

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Authors and Affiliations

  1. Department of Analytical and Medical Biochemistry and Microbiology, Voronezh State University, 394006, Voronezh, Russia

    Tatiana Popova, Larisa Matasova & Liliya Medvedeva

  2. ISOPlexis / BIOtecMOL, Centre of Macaronesian Studies, Madeira University, 9000-390, Funchal, Portugal

    Miguel A. A Pinheiro de Carvalho

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  1. Tatiana Popova
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  2. Miguel A. A Pinheiro de Carvalho
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  3. Larisa Matasova
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  4. Liliya Medvedeva
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Correspondence to Miguel A. A Pinheiro de Carvalho.

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Popova, T., Carvalho, M.A.A.P.d., Matasova, L. et al. Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia. Mol Cell Biochem 294, 97–105 (2007). https://doi.org/10.1007/s11010-006-9249-9

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  • DOI: https://doi.org/10.1007/s11010-006-9249-9

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