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Phylogeny and expression pattern of starch branching enzyme family genes in cassava (Manihot esculenta Crantz) under diverse environments

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Abstract

Starch branching enzyme (SBE) is one of the key enzymes involved in starch biosynthetic metabolism. In this study, six SBE family genes were identified from the cassava genome. Phylogenetic analysis divided the MeSBE family genes into dicot family A, B, C, and the new group. Tissue-specific analysis showed that MeSBE2.2 was strongly expressed in leaves, stems cortex, and root stele, and MeSBE3 had high expression levels in stem cortex and root stele of plants in the rapid growth stage under field condition, whereas the expression levels of MeSBE2.1, MeSBE4, and MeSBE5 were low except for in stems cortex. The transcriptional activity of MeSBE2.2 and MeSBE3 was higher compared with other members and gradually increased in the storage roots during root growth process, while the other MeSBE members normally remained low expression levels. Expression of MeSBE2.2 could be induced by salt, drought, exogenous abscisic acid, jasmonic acid, and salicylic acid signals, while MeSBE3 had positive response to drought, salt, exogenous abscisic acid, and salicylic acid in leaves but not in storage root, indicating that they might be more important in starch biosynthesis pathway under diverse environments.

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Acknowledgments

This study was supported by National Basic Research and Development Program (2010CB126600), China Agriculture Research System (CARS-12), and National international science and technology cooperation plan (2011DFB31690).

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Authors and Affiliations

  1. College of Agriculture, Hainan University, Renmin Road No. 58, Haikou, 570228, People’s Republic of China

    Jinli Pei

  2. Qiongzhou University, Sanyan, 572022, People’s Republic of China

    Huijun Wang

  3. The Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou, 571101, People’s Republic of China

    Jinli Pei, Zhiqiang Xia, Chen Liu, Xin Chen, Pingan Ma, Cheng Lu & Wenquan Wang

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Correspondence to Wenquan Wang.

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Fig.S1 Detailed information on the conserved motifs. Supplementary material 1 (JPG 1931 kb)

11010_2015_2445_MOESM2_ESM.jpg

Fig.S2 Expression profiles of MeSBE1 in leaves under various treatments. The relative expression variance was calculated as described in Methods and Materials, and cassava β-Actin gene was used to normalize the data as an internal control. Error bars were calculated based on four replicates. Different treatment and timing points were indicated in each table. Statistical difference (P<0.05) was indicated by different superscripts. A: MeSBE1 expression pattern in leaf under drought treatment. B: MeSBE1expression pattern in leaf under 200mM NaCl treatment. C: MeSBE1 expression pattern in leaf under high temperature treatment. D: MeSBE1expression pattern in leaf under cold treatment. E: MeSBE1expression pattern in leaf under 100uM abscisic acid (ABA) treatment. F: MeSBE1 expression pattern in leaf under 100uM jasmonic acid (JA) treatment. G: MeSBE1 expression pattern in leaf under 5mM salicylic acid (SA) treatment. Supplementary material 2 (JPG 583 kb)

11010_2015_2445_MOESM3_ESM.jpg

Fig.S3 Expression profiles of MeSBE2.1 in leaves under various treatments. The relative expression variance was calculated as described in Methods and Materials, and cassava β-Actin gene was used to normalize the data as an internal control. Error bars were calculated based on four replicates. Different treatment and timing points were indicated in each table. Statistical difference (P<0.05) was indicated by different superscripts. Supplementary material 3 (JPG 1437 kb)

11010_2015_2445_MOESM4_ESM.jpg

Fig.S4 Expression profiles of MeSBE3 in leaves under various treatments. The relative expression variance was calculated as described in Methods and Materials, and cassava β-Actin gene was used to normalize the data as an internal control. Error bars were calculated based on four replicates. Different treatment and timing points were indicated in each table. Statistical difference (P<0.05) was indicated by different superscripts. Supplementary material 4 (JPG 646 kb)

11010_2015_2445_MOESM5_ESM.jpg

Fig.S5 Expression profiles of MeSBE4 in leaves under various treatments. The relative expression variance was calculated as described in Methods and Materials, and cassava β-Actin gene was used to normalize the data as an internal control. Error bars were calculated based on four replicates. Different treatment and timing points were indicated in each table. Statistical difference (P<0.05) was indicated by different superscripts. Supplementary material 5 (JPG 634 kb)

11010_2015_2445_MOESM6_ESM.jpg

Fig.S6 Expression profiles of MeSBE5 in leaves under various treatments. The relative expression variance was calculated as described in Methods and Materials, and cassava β-Actin gene was used to normalize the data as an internal control. Error bars were calculated based on four replicates. Different treatment and timing points were indicated in each table. Statistical difference (P<0.05) was indicated by different superscripts. Supplementary material 6 (JPG 1477 kb)

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Pei, J., Wang, H., Xia, Z. et al. Phylogeny and expression pattern of starch branching enzyme family genes in cassava (Manihot esculenta Crantz) under diverse environments. Mol Cell Biochem 406, 273–284 (2015). https://doi.org/10.1007/s11010-015-2445-8

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