Abstract
Atherosclerosis has been linked to chronic inflammatory processes. Changes in the levels of lipoproteins, especially low-density lipoprotein or its variants, as well as inflammatory markers are risk factors for the atherosclerosis. In the present study, an experimental model of rheumatoid arthritis was developed by administrating collagen suspension intradermally in the tail region of Wistar albino rats. At the same time, a suspension of hesperidin (50 mg/kg body weight) and daidzein (20 mg/kg body weight) was orally administrated. The compounds were given in the morning and evening for 21 days. Levels of inflammatory markers in the homogenate of knee joints of experimental rats as well as plasma lipoproteins were investigated. The administration of hesperidin and daidzein caused significant (p < 0.001) decrease in articular elastase activity, TNF-α, and malondialdehyde levels. Further, arthritis scoring and histological findings supported the anti-inflammatory actions of the test compounds. Interestingly, the test compounds also lowered the plasma low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, and triglyceride but increased the level of high-density lipoprotein cholesterol. The test compounds thus ameliorated the risk factors of atherosclerosis. Furthermore, antioxidant roles of hesperidin as well as daidzein were evident from decrease in free radical load demonstrated as increase in total antioxidant level in plasma of arthritic animals treated with hesperidin and daidzein. In a separate in vitro experiment, enhanced free radical scavenging activity of hesperidin was demonstrated against 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid. The anti-inflammatory, hypolipidemic, and antioxidant actions of the naturally occurring test compounds, particularly hesperidin, seem to be quite effective against rheumatoid arthritis and atherosclerosis. Thus, their consumption may be helpful in prevention or at least delaying the onset of these diseases in susceptible individuals.
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Abbreviations
- ABTS·+ :
-
2,2-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid
- CAD:
-
Coronary artery disease
- CD:
-
Conjugated diene
- CHD:
-
Coronary heart disease
- CIAD:
-
Daidzein-Treated Rheumatoid Arthritis
- CIAH:
-
Hesperidin-Treated Rheumatoid Arthritis
- CIRA:
-
Collagen-Induced Rheumatoid Arthritis Control
- CTRL:
-
Normal Control
- CVD:
-
Cardiovascular disease
- DPPH· :
-
2,2-Diphenyl-1-picrylhydrazyl
- HDL-C:
-
High-density lipoprotein cholesterol
- Lb-LDL:
-
Large buoyant-LDL
- LDL:
-
Low-density lipoproteins
- MDA:
-
Malondialdehyde
- NOS:
-
Nitric oxide synthase
- RNS:
-
Reactive nitrogen species
- ROS:
-
Reactive oxygen species
- RA:
-
Rheumatoid arthritis
- Sd-LDL:
-
Small dense-LDL
- TBARS:
-
Thiobarbituric acid reactive substances
- TG:
-
Triglyceride
- TNF- α:
-
Tumor necrosis factor-α
- VLDL-C:
-
Very low-density lipoprotein cholesterol
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We acknowledge the Council of Science and Technology, Lucknow, India for providing financial assistance under the Young Scientist Project Scheme (Grant No. CST1239).
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Ahmad, S., Alam, K., Hossain, M.M. et al. Anti-arthritogenic and cardioprotective action of hesperidin and daidzein in collagen-induced rheumatoid arthritis. Mol Cell Biochem 423, 115–127 (2016). https://doi.org/10.1007/s11010-016-2830-y
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DOI: https://doi.org/10.1007/s11010-016-2830-y