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Chemically induced acute model of sarcosinemia in wistar rats

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Abstract

In the present study, we developed an acute chemically induced model of sarcosinemia in Wistar rats. Wistar rats of 7, 14 and 21 postpartum days received sarcosine intraperitoneally in doses of 0.5 mmol/Kg of body weight three time a day at intervals of 3 h. Control animals received saline solution (NaCl 0.85 g%) in the same volume (10 mL/Kg of body weight). The animals were killed after 30 min, 1, 2, 3 or 6 h after the last injection and the brain and the blood were collected for sarcosine measurement. The results showed that plasma and brain sarcosine concentrations achieved levels three to four times higher than the normal levels and decreased in a time-dependent way, achieving normal levels after 6 hours. Considering that experimental animal models are useful to investigate the pathophysiology of human disorders, our model of sarcosinemia may be useful for the research of the mechanisms of neurological dysfunction caused by high tissue sarcosine levels.

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Acknowledgments

This work was supported by the research grants from Programa de Núcleos de Excelência (PRONEX), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and FINEP Rede Instituto Brasileiro de Neurociência.

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  1. PPG Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90.035-003, Brazil

    Rodrigo Binkowski de Andrade, Tanise Gemelli, Denise Bertin Rojas, Carlos Severo Dutra-Filho & Clovis Milton Duval Wannmacher

  2. Universidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da Saúde, Departamento de Bioquímica, Rua Ramiro Barcelos 2600-Anexo, Porto Alegre, RS, 90035-003, Brazil

    Clovis Milton Duval Wannmacher

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  1. Rodrigo Binkowski de Andrade
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  2. Tanise Gemelli
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  3. Denise Bertin Rojas
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Correspondence to Clovis Milton Duval Wannmacher.

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de Andrade, R.B., Gemelli, T., Rojas, D.B. et al. Chemically induced acute model of sarcosinemia in wistar rats. Metab Brain Dis 31, 363–368 (2016). https://doi.org/10.1007/s11011-015-9759-9

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  • DOI: https://doi.org/10.1007/s11011-015-9759-9

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