Skip to main content
SpringerLink
Log in

Analysis of alien introgression in coffee tree (Coffea arabica L.)

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

The transfer of desired traits from related wild diploid Coffea species into the cultivated allotetraploid C. arabica is essential in coffee breeding to develop pest/disease-resistant cultivars. The present work is an attempt to gain insights into alien introgression in C. arabica. An F2 population derived from a cross between T5296 and Et6 was analysed with simple sequence repeat (SSR; microsatellite) and amplified fragment length polymorphism (AFLP) molecular markers. The T5296 is a derivative of an interspecific hybrid introgressed by the diploid C. canephora species and Et6 is a wild Ethiopian accession of C. arabica. The origin of the revealed polymorphism was determined by comparisons using representative accessions from C. arabica and its two diploid parental species, C. eugenioides and C. canephora. The number and mode of inheritance of canephora-introgressed segments were investigated, as well as their sub-genome localisation and rate of recombination. The results suggested that the transfer of desirable genes into C. arabica from C. canephora is not limited by the ploidy level differences or the suppression of recombination between the different genomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Anthony F, Combes MC, Astorga C, Bertrand B, Graziosi G, Lashermes P (2002) The origin of cultivated Coffea arabica L. revealed by AFLP and SSR markers. Theor Appl Genet 104:894–900

    Article  CAS  PubMed  Google Scholar 

  • Berthaud J (1978) L’hybridation interspécifique entre Coffea arabica L. et Coffea canephora Pierre. Obtention et comparaison des hybrides triploïdes, arabusta et hexaploïdes. Café Cacao Thé 22:87–109

    Google Scholar 

  • Bertrand B, Guyot B, Anthony F, Lashermes P (2003) Impact of introgression of Coffea canephora genes on the Arabica coffee cup quality. Theor Appl Genet 107:387–394

    Article  CAS  PubMed  Google Scholar 

  • Bettencourt A (1973) Cosideraçoes gerais sobre o “Hibrido de Timor”. Instituto Agronomico de Campinas, Circular No. 31, Campinas, p 285

  • Burnham CR (1962) Discussions in cytogenetics. Burgess Publishing, Minneapolis

    Google Scholar 

  • Combes MC, Andrzejewski S, Anthony F, Bertrand B, Rovelli P, Graziosi G, Lashermes P (2000) Characterisation of microsatellite loci in Coffea arabica and related coffee species. Mol Ecol 9:1178–1180

    Article  CAS  PubMed  Google Scholar 

  • Davis AP, Rakotonasolo F (2008) A taxonomic revision of the baracoffea alliance: nine remarkable Coffea species from western Madagascar. Bot J Linn Soc 158:355–390

    Article  Google Scholar 

  • Davis AP, Govaerts R, Bridson DM, Stoffelen P (2006) An annotated taxonomic conspectus of the genus Coffea (Rubiaceae). Bot J Linn Soc 152:465–512

    Article  Google Scholar 

  • Dussert S, Lashermes P, Anthony F, Montagnon C, Trouslot P, Combes MC, Berthaud J, Noirot M, Hamon S (2003) Coffee (Coffea canephora). In: Hamon P, Seguin M, Perrier X, Glaszmann JC (eds) Genetic diversity of cultivated tropical plants. CIRAD, Paris, pp 239–258

    Google Scholar 

  • Gichuru EK, Agwanda CO, Combes MC, Mutitu EW, Ngugi ECK, Bertrand B, Lashermes P (2008) Identification of molecular markers linked to a gene conferring resistance to coffee berry disease (Colletotrichum kahawae) in Coffea arabica L. Plant Pathol 57:1117–1124

    Article  CAS  Google Scholar 

  • Grassias M (1980) Etude de la fertilité et du comportement méiotique des hybrides interspécifiques Arabusta Coffea arabica x C. canephora (Thesis). University of Paris XI (Orsay), France

  • Hajjar R, Hodgkin T (2007) The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica 156:1–13

    Article  Google Scholar 

  • Haldane JBS (1930) Theoretical genetics of autopolyploids. J Genet 22:359–372

    Article  Google Scholar 

  • Herrera JC, Combes MC, Anthony F, Charrier A, Lashermes P (2002) Introgression into the allotetraploid coffee (Coffea arabica L.): segregation and recombination of the C. canephora genome in the tetraploid interspecific hybrid (C. arabica × C. canephora). Theor Appl Genet 104:661–668

    Article  CAS  PubMed  Google Scholar 

  • Herrera JC, Combes MC, Cortina H, Lashermes P (2004) Factors regulating gene introgression into the allotetraploid Coffea arabica L. from its diploid relatives. Genome 47:1053–1060

    Article  CAS  PubMed  Google Scholar 

  • Herrera JC, Alvarado G, Cortina H, Combes MC, Romero G, Lashermes P (2009) Genetic analysis of partial resistance to coffee leaf rust (Hemileia vastatrix Berk & Br.) introgressed into the cultivated Coffea arabica L. from the diploid C. canephora species. Euphytica 167:57–67

    Article  Google Scholar 

  • Ji Y, Chetelat RT (2003) Homoeologous pairing and recombination in Solanum lycopersicoides monosomic addition and substitution lines of tomato. Theor Appl Genet 106:979–989

    CAS  PubMed  Google Scholar 

  • Krug CA, Mendes AJT (1940) Cytological observations in Coffea - IV. J Genet 39:189–203

    Article  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Bralow A, Daly MJ, Loincoln SE, Newburg L (1987) MAP MAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Lashermes P, Combes MC, Robert J, Trouslot P, D’hont A, Anthony F, Charrier A (1999) Molecular characterisation and origin of the Coffea arabica L. genome. Mol Gen Genet 261:259–266

    Article  CAS  PubMed  Google Scholar 

  • Lashermes P, Andrzejewski S, Bertrand B, Combes MC, Dussert S, Grasiozi G, Trouslot P, Anthony F (2000a) Molecular analysis of introgressive breeding in coffee (Coffea arabica). Theor Appl Genet 100:139–146

    Article  CAS  Google Scholar 

  • Lashermes P, Paczek V, Trouslot P, Combes MC, Couturon E, Charrier A (2000b) Single-locus inheritance in the allotetraploid Coffea arabica L. and interspecific hybrid C. arabica × C. canephora. J Hered 91:81–85

    Article  CAS  PubMed  Google Scholar 

  • Lashermes P, Combes MC, Prakash NS, Trouslot P, Lorieux M, Charrier A (2001) Genetic linkage map of Coffea canephora: effect of segregation distortion and analysis of recombination rate in male and female meioses. Genome 44:589–595

    Article  CAS  PubMed  Google Scholar 

  • Lashermes P, Bertrand B, Etienne H (2009) Breeding Coffee (Coffea arabica) for sustainable production. In: Jain SM, Priyadarshan PM (eds) Breeding plantation tree crops. Springer, New York, pp 525–544

    Google Scholar 

  • Mahé L, Le Pierrès D, Combes MC, Lashermes P (2007) Introgressive hybridization between the allotetraploid Coffea arabica and one of its diploid ancestors, Coffea canephora, in an exceptional sympatric zone in New Caledonia. Genome 50:316–324

    Article  PubMed  Google Scholar 

  • Mardsen JE, Schwager SJ, May B (1987) Single locus inheritance in the tetraploid treefrog Hyla versicolor with an analysis of expected progeny ratios in tetraploid organisms. Genetics 116:299–311

    Google Scholar 

  • Neu C, Stein N, Keller B (2002) Genetic mapping of the Lr20–Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45:737–744

    Article  CAS  PubMed  Google Scholar 

  • Noir S, Anthony F, Bertrand B, Combes MC, Lashermes P (2003) Identification of a major gene (Mex-1) from Coffea canephora conferring resistance to Meloidogyne exigua in coffee. Plant Pathol 52:97–103

    Article  CAS  Google Scholar 

  • Owuor JBO (1985) Interspecific hybridization between Coffea arabica L. and tetraploid C. canephora P. ex Fr. II. Meiosis in F1 hybrids and backcrosses to C. arabica. Euphytica 34:355–360

    Article  Google Scholar 

  • Paterson AH, Tanksley SD, Sorrells ME (1991) DNA markers in plant improvement. Adv Agron 46:39–90

    Article  CAS  Google Scholar 

  • Puchta H, Hohn B (1996) From centiMorgans to base pairs: homologous recombination in plants. Trends Plant Sci 1:340–348

    Google Scholar 

  • Rieseberg LH, Baird SJE, Gardner KA (2000) Hybridization, introgression, and linkage evolution. Plant Mol Biol 42:205–224

    Article  CAS  PubMed  Google Scholar 

  • Stebbins GL (1950) Variation and evolution in plants. Colombia Univ. Press, New York

    Google Scholar 

  • Stebbins GL (1958) The inviability, weakness and sterility in interspecific hybrids. Adv Genet 9:147–215

    Article  CAS  PubMed  Google Scholar 

  • Stirling B, Newcombe G, Vrebalov J, Bosdet I, Bradshaw HD (2001) Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust. Theor Appl Genet 103:1129–1137

    Article  CAS  Google Scholar 

  • Tanksley SD, McCouch SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066

    Article  CAS  PubMed  Google Scholar 

  • Van der Vossen HAM (2001) Coffee breeding practices. In: Clarke RJ, Vitzthum OG (eds) Coffee. Recent developments–agronomy, vol 1. Blackwell Science Ltd, London, pp 184–201

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut de Recherche pour le Développement (IRD), UMR RPB (CIRAD, IRD, UM2), BP 64501, 34394, Montpellier Cedex 5, France

    Philippe Lashermes, Marie-Christine Combes, Caroline Ansaldi, Elijah Gichuru & Sandra Noir

  2. Coffee Research Foundation (CRF), P O Box 4, 00232, Ruiru, Kenya

    Elijah Gichuru

  3. School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK

    Sandra Noir

Authors
  1. Philippe Lashermes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marie-Christine Combes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Caroline Ansaldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Elijah Gichuru
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sandra Noir
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Philippe Lashermes.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lashermes, P., Combes, MC., Ansaldi, C. et al. Analysis of alien introgression in coffee tree (Coffea arabica L.). Mol Breeding 27, 223–232 (2011). https://doi.org/10.1007/s11032-010-9424-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-010-9424-2

Keywords

Search

Navigation