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Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding

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Abstract

Rice blast is one of the most serious diseases in rice (Oryza sativa L.) worldwide. Jin 23B is the maintainer line, a parent for a number of hybrid rice varieties used widely in China. However, Jin 23B is highly susceptible to rice blast. In this study, Pi1, Pi2, and D12 were introgressed to improve the blast resistance of Jin 23B and its derived hybrids, Jinyou 402 and Jinyou 207, by marker-assisted selection (MAS). The improved Jin 23B, which carried single, two, and three genes, were evaluated for their resistance to rice blast using natural inoculation methods in disease nursery of Xianfeng, Hubei, China. The results showed that, the greater the number of genes contained in the improved Jin 23B and hybrids, the higher the resistance to rice blast. Pi1, Pi2, and D12 showed a strong dosage effect on the resistance to blast in the hybrid background during the entire growth duration in the field condition, being very useful for breeding blast-resistant hybrids. The result of examining agronomic traits showed that the improved Jin 23B and its derived hybrid rice were taller than or similar to controls, when there was no disease stress.

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Acknowledgments

We thank IRRI for providing the cross parent BL6. This work was partially supported by grants from the National High Technology Research (2012AA101102), the National Program on R & D of Transgenic Plants of China (2011ZX08001-001, 2011ZX08001-002), the foundation of the Ministry of Agriculture (CARS-01-03, 2011-Z66), and the National Natural Science Foundation of China.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research and National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan, 430070, China

    Haichao Jiang, Yutao Feng, Liang Bao, Xin Li, Guanjun Gao, Qinglu Zhang, Jinghua Xiao, Caiguo Xu & Yuqing He

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  1. Haichao Jiang
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  2. Yutao Feng
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  3. Liang Bao
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  4. Xin Li
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  5. Guanjun Gao
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  6. Qinglu Zhang
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  7. Jinghua Xiao
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  8. Caiguo Xu
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  9. Yuqing He
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Correspondence to Yuqing He.

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Jiang, H., Feng, Y., Bao, L. et al. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding. Mol Breeding 30, 1679–1688 (2012). https://doi.org/10.1007/s11032-012-9751-6

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  • DOI: https://doi.org/10.1007/s11032-012-9751-6

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