Abstract
Future barley cultivars will have to produce under the constraints of higher temperature in combination with increased concentrations of atmospheric carbon dioxide and ozone as a consequence of climate change. A diverse set of 167 spring barley genotypes was cultivated under elevated levels of temperature (+5 °C) and [CO2] (700 ppm) as single factors and in combination as well as under elevated [O3] (100–150 ppb) as single factor. The setting in general resembled changes projected by IPCC (AR5) to take place at the end of this century. A genome-wide association study (GWAS) was performed to identify markers for increased primary production under climate change conditions and reveal possible genes of interest. Phenotyped traits included grain yield, number of grains, number of ears per plant, aboveground vegetative biomass, harvest index and stability of the production parameters over the five applied treatments. The GWAS encompassed 7864 SNP markers (Illumina iselect), a compressed mixed linear model with the GAPIT package, and conservative validation of markers. A total of 60 marker-trait associations [−log10(P value) 2.97–5.58] were identified, e.g. grain yield under elevated temperature on barley chromosome 2H, static stability of grain yield on 7H, sites for exploitation of elevated [CO2] on 4H and 7H and associations under the two-factor treatment. Marker-trait associations identified from single-factor treatments were not retrieved, when elevated [CO2] and temperature were combined emphasizing the need for multifactor experiments. This GWA study identified markers and chromosome regions to be targeted in breeding for development of climate resilient cultivars.
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Abbreviations
- AllM:
-
Dataset of all markers
- BM:
-
Aboveground vegetative biomass
- EG:
-
Number of ears with grains
- ET:
-
Number of ears
- GN:
-
Number of grains
- GWAS:
-
Genome-wide association study
- GY:
-
Grain yield
- HI:
-
Harvest index
- LD:
-
Linkage disequilibrium
- MwP:
-
Markers from the AllM dataset with identified position
- QTL:
-
Quantitative trait loci
- SNP:
-
Single nucleotide polymorphism
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Acknowledgments
We thank all involved in the cultivation and harvest procedures and Allan Murphy and Esben Højrup for technical assistance in RERAF during the experiment. The accessions were provided by NordGen (the Nordic Genetic Resource Center; http://www.nordgen.org/), breeders of the Nordic network ‘Sustainable primary production in a changing climate’ (NordForsk) and a few came from the BAR-OF project (ICROFS, Denmark). The CO2 used in this study was generously supplied by Air Liquide Danmark A/S. The Nordic Council of Ministers supported the major part of this research through the network, ‘Sustainable primary production in a changing climate’. Other funders were The Danish Council for Independent Research: Technology and Production Sciences (FTP) via the project ‘Climate Change Effects on Plant Health’ (project 0602-00589B), and COBRA (Core Organic II).
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Ingvordsen, C.H., Backes, G., Lyngkjær, M.F. et al. Genome-wide association study of production and stability traits in barley cultivated under future climate scenarios. Mol Breeding 35, 84 (2015). https://doi.org/10.1007/s11032-015-0283-8
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DOI: https://doi.org/10.1007/s11032-015-0283-8