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Identification and mapping of two adult plant leaf rust resistance genes in durum wheat

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Abstract

The International Maize and Wheat Improvement Center (CIMMYT)-derived durum wheat line Quetru is moderately resistant to leaf rust and displays disease severities of 15–30% at the adult plant stage. Atil C2000 is moderately susceptible to leaf rust at adult plant stage and displays severities of 20–60%. These two durum wheats were crossed with the susceptible line Atred#1, and the respective recombinant inbred line populations were phenotyped for resistance to leaf rust in field trials in four environments in Mexico. The phenotypic segregation in the Atred#1 × Atil C2000 population was attributed to the pleiotropic adult plant resistance (APR) gene Lr46 based on the closest marker cslv46G22 located within 1.4 cM. Lr46 reduced leaf rust severity by 19.6–35.5%, and it was also identified in the Atred#1 × Quetru population, where it reduced leaf rust severity by 28.5–57.7% depending on the environment. We also identified a putatively novel, race-specific APR gene LrQ in Quetru that is located on the Chromosome 6BL and flanked by SNP markers AX-95144243 and AX-95155193. These two markers have been converted into kompetitive allele–specific PCR (KASP) markers and validated in the population. LrQ was ineffective at the seedling stage but reduced leaf rust severity by 37.3% on average over four field trials. The combination of LrQ and Lr46 resulted in significantly reduced leaf rust severity in the field and could be used to breed improved durum wheat varieties.

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Acknowledgments

We appreciate the technical editing by CIMMYT Communication Unit.

Funding

This work was supported by the Australian Grains Research and Development Corporation (GRDC) with funding to the Australian Cereal Rust Control Program (ACRCP), CGIAR Research Program WHEAT (CRP-WHEAT), International Cooperation and Exchange of the National Natural Science Foundation of China (Grant No. 31861143010), and Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (Grant No. 2662019PY009). The SNP genotyping was supported by CRP partnership grant A4031.09.46 between Trait Genetics GmbH and CIMMYT/ICARDA.

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Author notes

  1. Caixia Lan and Zhikang Li contributed equally to this work.

Authors and Affiliations

  1. College of Plant Science & Technology, Huazhong Agricultural University, No. 1, Shizishan Street, Hongshan District, Wuhan City, 430070, Hubei Province, People’s Republic of China

    Caixia Lan & Zhikang Li

  2. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico D.F, Mexico

    Sybil A. Herrera-Foessel, Bhoja R. Basnet, Susanne Dreisigacker & Ravi P. Singh

  3. Campo Experimental Valle de México INIFAP, Apdo. Postal 10, 56230, Chapingo, Edo. de México, Mexico

    Julio Huerta-Espino

  4. Mianyang Institute of Agricultural Science/Mianyang Branch of National Wheat Improvement Center, 8 Songjiang Road, Mianyang, 621023, Sichuan Province, People’s Republic of China

    Yong Ren

  5. CSIRO Agriculture & Food, GPO Box 1600, Canberra, ACT, 2601, Australia

    Evans Lagudah

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  1. Caixia Lan
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  2. Zhikang Li
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Contributions

Caixia Lan phenotyped the population, did analysis, and wrote the manuscript.

Zhikang Li and Susanne Dreisigacker genotyped the population.

Sybil A. Herrera-Foessel, Bhoja R. Basnet, Yong Ren, and Evans Lagudah phenotyped the population and reviewed the manuscript.

Ravi P. Singh and Julio Huerta-Espino developed and phenotyped the population.

Corresponding authors

Correspondence to Caixia Lan or Ravi P. Singh.

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Fig. S1

The leaf rust response of Atred#1 and Atil C2000 at the adult plant stage in the field. (JPG 272 kb)

Fig. S2

Clustering pattern of two KASP markers AX95144243 that are closely linked to LrQ genotypes of 6 lines with two replications. (JPG 58 kb)

Fig. S3

Clustering pattern of two KASP markers 2277143 (A) and 1234305 (B) that are closely linked to QLr.cim-6BL from Atred×Bairds population genotypes of 6 lines with two replications. (JPG 57 kb)

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Lan, C., Li, Z., Herrera-Foessel, S.A. et al. Identification and mapping of two adult plant leaf rust resistance genes in durum wheat. Mol Breeding 39, 118 (2019). https://doi.org/10.1007/s11032-019-1024-1

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