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Differential display reveals a novel pig gene, PRPF3, which is differentially expressed in Large White versus Wujin skeletal muscle tissues

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Abstract

The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends method. The open reading frame of this gene encodes a protein of 683 amino acids, which is homologous with the PRP3 pre-mRNA processing factor 3 (PRPF3) of five species: bovine (99%), human (99%), sumatran orangutan (99%), mouse (99%) and chicken (94%). This newly identified gene was respectively defined as the swine PRPF3 gene. This gene is structured in 15 exons and 14 introns as revealed by computer-assisted analysis. The phylogenetic tree analysis revealed that the swine PRPF3 gene has a closer genetic relationship with the PRPF3 gene of sumatran orangutan.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30800810).

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  1. College of Animal Sciences and Technology, Yunnan Agricultural University, 650201, Kunming, China

    Liu Yonggang

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  1. Liu Yonggang
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Correspondence to Liu Yonggang.

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Yonggang, L. Differential display reveals a novel pig gene, PRPF3, which is differentially expressed in Large White versus Wujin skeletal muscle tissues. Mol Biol Rep 37, 2687–2692 (2010). https://doi.org/10.1007/s11033-009-9799-5

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  • DOI: https://doi.org/10.1007/s11033-009-9799-5

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