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Effects of myeloperoxidase −463 G/A gene polymorphism and plasma levels on coronary artery disease

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Abstract

Myeloperoxidase is a lysosomal enzyme of polymorphonuclear leucocytes that contributes to inflamatory responses. In previous studies it was shown that MPO was synthesized in atherosclerotic lesions responsible of lipoprotein oxidations. We aimed to determine the MPO −463 G/A gene polymorphism distribution in Turkish population and evaluate the effects of it on myeloperoxidase levels. There were 100 myocardial infarct patients and 100 healthy control subjects in our study. MPO polymorphism was studied by using PCR-RFLP technique and MPO levels were measured by ELISA. It was shown that MPO levels were increasing in patients after myocardial infarct event but there were no effect of MPO −463 G/A polymorphism on MPO levels. It was also found that serum total cholesterol and LDL-cholesterol levels and smoking was contributing factors in increments of MPO enzymes. We observed that MPO levels were increased in CAD but there were no effect of MPO −463 G/A polymorphism on MPO levels.

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Acknowledgements

This study was supported by TUBITAK as project no: 107S289.

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Authors and Affiliations

  1. Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, 34280, Capa Istanbul, Turkey

    Arzu Ergen & Özlem Timirci

  2. Department of Cardiovascular Surgery, Faculty of Medicine, University of Marmara, Istanbul, Turkey

    Selim İsbir

  3. Uskudar State Hospital, Clinic of Cardiovascular Surgery, Istanbul, Turkey

    Atike Tekeli

  4. Department of Medical Biology, Yeditepe University, Istanbul, Turkey

    Turgay İsbir

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  1. Arzu Ergen
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  2. Selim İsbir
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  3. Özlem Timirci
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Correspondence to Arzu Ergen.

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Ergen, A., İsbir, S., Timirci, Ö. et al. Effects of myeloperoxidase −463 G/A gene polymorphism and plasma levels on coronary artery disease. Mol Biol Rep 38, 887–891 (2011). https://doi.org/10.1007/s11033-010-0181-4

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  • DOI: https://doi.org/10.1007/s11033-010-0181-4

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