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Novel SNPs in the caprine stearoyl-CoA desaturase (SCD) and decorin (DCN) genes that are associated with growth traits in Chinese goat breeds

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Abstract

Stearoyl-CoA desaturase (SCD) is an iron-containing enzyme involving in the biosynthesis of monounsaturated fatty acids (MUFA) in mammary gland and adipose tissue, while decorin (DCN) consists of a protein core and a single dermatan or chondroitin sulfate glycosaminoglycan chain, contributing multifunctionally to matrix assembly, modulation of the activity of growth factors and cell migration and proliferation. However, few studies have focused on the genetic variability of them in goat. Herein, five Chinese goat breeds (1229 animals) were analyzed. Based on DNA pooling and PCR-RFLP, three nucleotide substitutions, one of which caused a amino acid substitution, were detected in SCD gene and three haploids (A, B, C) were constructed. According to SSCP analysis and DNA sequencing methods, a 2-bp deletion and two other SNPs were found existing in another analyzed gene DCN, and three haploids (X, Y, Z) were built. Associations between the genotypes and the growth traits (body length, body height, chest circumference, cannon circumference) were also analyzed. For SCD gene, genotype CC individuals had significant greater body height in Guanzhong and body length in both Guanzhong and Xinong saanen than genotype BC individuals (P < 0.05). For DCN gene, individuals with genotype XX was obviously higher than that with genotype XY (P < 0.05). These results indicated that genotype CC of SCD gene and genotype XX of DCN gene could be used for the breeding of new breeds of goat in China.

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Acknowledgements

This work was supported by the National “863” Program of the P.R. China (No. 2008AA10Z138), the “13115” Sci-Tech innovation program of Shaanxi province (2008ZDKG-11), Natural Science Foundation of Jiangsu Province (No. BK2008120), Research Fund for the Doctor Program of Higher Education of China (No. 20080712001), Talents Foundation of Northwest A&F University (No. 01140411), The Young Topnotch Researcher Support Project of Northwest A&F University (No.QNGG-2009-007).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, No.22 Xinong Road, Yangling, 712100, Shaanxi, China

    Zhongqi Chen, Zhuanjian Li, Xianyong Lan, Yujiao Qu, Yanli Liu, Chuzhao Lei & Hong Chen

  2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, 221116, Jiangsu, China

    Jiajie Sun, Chunlei Zhang, Xingtang Fang & Hong Chen

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  1. Zhongqi Chen
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Correspondence to Hong Chen.

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Zhongqi Chen and Jiajie Sun contribute equally to this work.

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Chen, Z., Sun, J., Li, Z. et al. Novel SNPs in the caprine stearoyl-CoA desaturase (SCD) and decorin (DCN) genes that are associated with growth traits in Chinese goat breeds. Mol Biol Rep 38, 3121–3127 (2011). https://doi.org/10.1007/s11033-010-9982-8

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