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BRCA1: a new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score

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Abstract

Bovine mastitis is a very complex and common disease of dairy cattle and a major source of economic losses to the dairy industry worldwide. In this study, the bovine breast cancer 1, early onset gene (BRCA1) was taken as a candidate gene for mastitis resistance. The main object of this study was to investigate whether the BRCA1 gene was associated with mastitis in cattle. Through DNA sequencing, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Created Restriction Site PCR (CRS-PCR) methods, three SNPs (G22231T, T25025A, and C28300A) were detected and twenty-four combinations of these SNPs were observed. The single SNP and their genetic effects on somatic cell score (SCS) were evaluated and a significant association with SCS was found in C28300A. The mean of genotype EE was significantly lower than those of genotypes EF and FF. The results of combined genotypes analysis of three SNPs showed that BBDDFF genotype with the highest SCS were easily for the mastitis susceptibility, whereas AACCEE genotype with the lowest SCS were favorable for the mastitis resistance. The information provided in the present study will be very useful for improving mastitis resistance in dairy cattle by marker-assisted selection.

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Acknowledgment

The study was financially supported by the New Variety of Transgenic Organism Great Breeding Program (NO.2008ZX08007-002 and NO.2009ZX08009-157B) and the earmarked fund for Modern Agro-industry Technology Research System (No.CARS-38); National Natural Science Foundation of China (81102746), Beijing Natural Science Foundation (5113033), Scientific Research Foundation of the State Human Resource Ministry and the Education Ministry for Returned Chinese Scholars, New Star Project of Peking Union Medical College, Youth Foundation of Peking Union Medical College, the Research Fund for the Doctoral Program of Higher Education (20111106120028), “Major Drug Discovery” major science and technology research “12nd Five-Year Plan” (2012ZX09301-002-001) China Medical Board of New York (A2009001) granted to Zhihua Liu.

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Authors and Affiliations

  1. Center for Computational Biology and Bioinformatics, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, People’s Republic of China

    Zhengrong Yuan, Guiyan Chu, Yang Dan & Zhihua Liu

  2. Laboratory of Molecular Biology and Bovine Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Jiao Li, Lupei Zhang, Xue Gao, Huijiang Gao, Junya Li & Shangzhong Xu

Authors
  1. Zhengrong Yuan
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  2. Guiyan Chu
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  3. Yang Dan
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  4. Jiao Li
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  5. Lupei Zhang
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  6. Xue Gao
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  7. Huijiang Gao
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  8. Junya Li
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  9. Shangzhong Xu
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  10. Zhihua Liu
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Correspondence to Shangzhong Xu or Zhihua Liu.

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Z. Yuan, G. Chu and Y. Dan have contributed equally to this work.

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Yuan, Z., Chu, G., Dan, Y. et al. BRCA1: a new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score. Mol Biol Rep 39, 6625–6631 (2012). https://doi.org/10.1007/s11033-012-1467-5

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  • DOI: https://doi.org/10.1007/s11033-012-1467-5

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