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Bioceramic-collagen scaffolds loaded with human adipose-tissue derived stem cells for bone tissue engineering

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Abstract

The combination of bioceramics and stem cells has attracted the interest of research community for bone tissue engineering applications. In the present study, a combination of Bio-Oss® and type 1 collagen gel as scaffold were loaded with human adipose-tissue derived mesenchymal stem cells (AT-MSCs) after isolation and characterization, and the capacity of them for bone regeneration was investigated in rat critical size defects using digital mammography, multi-slice spiral computed tomography imaging and histological analysis. 8 weeks after implantation, no mortality or sign of inflammation was observed in the site of defect. According to the results of imaging analysis, a higher level of bone regeneration was observed in the rats receiving Bio-Oss®-Gel compared to untreated group. In addition, MSC-seeded Bio-Oss-Gel induced the highest bone reconstruction among all groups. Histological staining confirmed these findings and impressive osseointegration was observed in MSC-seeded Bio-Oss-Gel compared with Bio-Oss-Gel. On the whole, it was demonstrated that combination of AT-MSCs, Bio-Oss and Gel synergistically enhanced bone regeneration and reconstruction and also could serve as an appropriate structure to bone regenerative medicine and tissue engineering application.

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Authors and Affiliations

  1. Departments of Anatomy and Cell Biology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Neda Daei-farshbaf, Abbas Piryaei, Fatemeh Fadaei Fathabady, Hamid Nazarian & Mohsen Norouzian

  2. Departments of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran

    Abdolreza Ardeshirylajimi & Sadegh-Lotfalah Moradi

  3. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

    Ehsan Seyedjafari

  4. Endocrine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mehdi Hedayati

  5. Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad Salehi

  6. Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

Authors
  1. Neda Daei-farshbaf
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  2. Abdolreza Ardeshirylajimi
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  3. Ehsan Seyedjafari
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  8. Masoud Soleimani
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  10. Sadegh-Lotfalah Moradi
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  11. Mohsen Norouzian
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Correspondence to Mohsen Norouzian.

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Daei-farshbaf, N., Ardeshirylajimi, A., Seyedjafari, E. et al. Bioceramic-collagen scaffolds loaded with human adipose-tissue derived stem cells for bone tissue engineering. Mol Biol Rep 41, 741–749 (2014). https://doi.org/10.1007/s11033-013-2913-8

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  • DOI: https://doi.org/10.1007/s11033-013-2913-8

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