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Characterization of nineteen microsatellite markers and development of multiplex PCRs for the wedge clam Donax trunculus (Mollusca: Bivalvia)

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Abstract

The wedge clam Donax trunculus is an Atlantic-Mediterranean warm-temperate species found from Senegal to the northern coast of France, including the Mediterranean and Black Sea. It is commercially exploited in several European countries and constitutes an important fishing resource due to its high economical value. To contribute to its conservation and management, nineteen microsatellite markers were isolated from two enriched genomic libraries. These loci were characterized in 30 clams from a single population from northwest Spain. The number of alleles per locus ranged from 2 to 17 and observed and expected heterozygosity varied from zero to 0.714 and from 0.078 to 0.950, respectively. Linkage disequilibrium was not detected and nine loci were in agreement with Hardy–Weinberg equilibrium. Fifteen polymorphic markers were arranged into three multiplex PCR sets to reduce both time and cost of microsatellite genotyping. This is the first time that polymorphic microsatellite markers have been reported for D. trunculus. These new markers provide a valuable resource for future population genetics studies and management and culture of this species.

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Acknowledgments

We thank Dra. Dorotea Martínez and Susana Nóvoa from Centro de Cultivos Marinos de Ribadeo—CIMA (Xunta de Galicia) for supplying the wedge clam samples, Jose García Gil for his technical assistance and Luisa Martínez for helpful comments on earlier versions of this manuscript. We would also like to thank Dr. James D. Austin for their revision of English grammar and style. This work was supported by the Consellería de Economía e Industria (Xunta de Galicia) through project 10MMA103013 and by Ministerio de Educación through a predoctoral FPU fellowship awarded to Ana Nantón.

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  1. Grupo Xenomar, Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus de A Coruña, 15071 A Coruña, Spain

    Ana Nantón, Alberto Arias-Pérez, Josefina Méndez & Ruth Freire

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  1. Ana Nantón
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  2. Alberto Arias-Pérez
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  3. Josefina Méndez
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  4. Ruth Freire
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Correspondence to Ana Nantón.

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Nantón, A., Arias-Pérez, A., Méndez, J. et al. Characterization of nineteen microsatellite markers and development of multiplex PCRs for the wedge clam Donax trunculus (Mollusca: Bivalvia). Mol Biol Rep 41, 5351–5357 (2014). https://doi.org/10.1007/s11033-014-3406-0

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