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The effects of the Penicillium mycotoxins citrinin, cyclopiazonic acid, ochratoxin A, patulin, penicillic acid, and roquefortine C on in vitro proliferation of porcine lymphocytes

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Abstract

The in vitro effect of each of the Penicillium mycotoxins citrinin (CIT), cyclopiazonic acid (CPA), ochratoxin A (OTA), patulin (PAT), penicillic acid (PIA) and roquefortine C (RQC) on mitogen induced lymphocyte proliferation was determined using purified lymphocytes from 6 piglets. Dose response curves for each mycotoxin were generated and the concentrations producing 50% inhibition of cell proliferation (IC50) were estimated. OTA and PAT were the most potent toxins with IC50 of 1.3 and 1.2 µmol/l, respectively (0.52 and 0.18 mg/l, respectively). Based on molar concentrations, OTA was 15, 30, 40, and 65 times more potent as an inhibitor than PIA, CIT, CPA and RQC, respectively.

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Authors and Affiliations

  1. National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway

    Modestas Keblys, Aksel Bernhoft, Constance C. Höfer, Ellen Morrison & Arne Flåøyen

  2. Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033, Oslo, Norway

    Hans Jørgen S. Larsen

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  1. Modestas Keblys
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  2. Aksel Bernhoft
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  3. Constance C. Höfer
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  5. Hans Jørgen S. Larsen
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Correspondence to Aksel Bernhoft.

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Keblys, M., Bernhoft, A., Höfer, C.C. et al. The effects of the Penicillium mycotoxins citrinin, cyclopiazonic acid, ochratoxin A, patulin, penicillic acid, and roquefortine C on in vitro proliferation of porcine lymphocytes. Mycopathologia 158, 317–324 (2004). https://doi.org/10.1007/s11046-005-5523-8

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  • DOI: https://doi.org/10.1007/s11046-005-5523-8

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