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Changes in the Immunohistochemical Characteristics of Neurons in a Number of Hypothalamic Nuclei on Aging

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Objectives. To identify changes in the immunohistochemical characteristics of neurons in the arcuate (AN), ventromedial (VMN), and dorsomedial (DMN) nuclei of the hypothalamus in rats on aging. Materials and methods. Studies were performed using male Wistar rats aged 3–4 months (five individuals) and 12–18 months (five individuals) using immunohistochemical methods. Results. Aging was associated with a decrease in the relative content of calbindin-immunopositive neurons in the DMN, while calretinin-immunopositive neurons in the DMN and VMN increased. The numbers of neurons immunopositive for neuronal nitric oxide synthase and the level of immunofluorescence for this enzyme were greater in all the nuclei studied in older animals. Older rats also showed a higher density of neuropeptide Y-immunopositive fibers in the VMN, with a smaller number in the DMN. Conclusions. Aging was associated with changes in the neurochemical composition of neurons, mainly in the ventromedial and dorsomedial nuclei of the hypothalamus.

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References

  1. P. M. Masliukov, A. D. Nozdrachev, and A. I. Emanuilov, “Developmental characteristics of the expression of calcium-binding proteins in neurons in the ganglia of the autonomic nervous system,” Usp. Gerontol., 29, No. 2, 247–253 (2016).

    CAS  Google Scholar 

  2. J. H. Ahn, S. Hong, J. H. Park, et al., “Immunoreactivities of calbindin D28k, calretinin and parvalbumin in the somatosensory cortex of rodents during normal aging,” Mol. Med. Rep., 16, No. 5, 7191–7198 (2017), doi: https://doi.org/10.3892/mmr.2017.7573.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. K. Chachlaki, J. Garthwaite, and V. Prevot, “The gentle art of saying NO: how nitric oxide gets things done in the hypothalamus,” Nat. Rev. Endocrinology, 13, No. 9, 521–535 (2017), doi: https://doi.org/10.1038/nrendo.2017.69.

    Article  CAS  PubMed  Google Scholar 

  4. P. A. Dacks, C. L. Moreno, E. S. Kim, et al., “Role of the hypothalamus in mediating protective effects of dietary restriction during aging,” Front. Neuroendocrinol., 34, No. 2, 95–106 (2013), doi: https://doi.org/10.1016/j.yfrne.2012.12.001.

    Article  CAS  PubMed  Google Scholar 

  5. V. M. Dilman and V. N. Anisimov, “Hypothalamic mechanisms of ageing and of specific age pathology – I. Sensitivity threshold of hypothalamo-pituitary complex to homeostatic stimuli in the reproductive system,” Exp. Gerontol., 14, 161–174 (1979).

    Article  CAS  Google Scholar 

  6. M. Ferrini, C. Wang, R. S. Swerdloff, et al., “Aging-related increased expression of inducible nitric oxide synthase and cytotoxicity markers in rat hypothalamic regions associated with male reproductive function,” Neuroendocrinology, 74, No. 1, 1–11 (2001).

    Article  CAS  Google Scholar 

  7. K. Kim and H. K. Choe, “Role of hypothalamus in aging and its underlying cellular mechanisms,” Mech. Aging Dev., 177, 74–79 (2019), doi: https://doi.org/10.1016/j.mad.2018.04.008.

    Article  CAS  PubMed  Google Scholar 

  8. P. M. Masliukov, A. I. Emanuilov, K. Moiseev, et al., “Development of noncatecholaminergic sympathetic neurons in para- and prevertebral ganglia of cats,” Int. J. Dev. Neurosci., 40, 76–84 (2015).

    Article  CAS  Google Scholar 

  9. M. P. Mattson, “Calcium and neurodegeneration,” Aging Cell, 6, No. 3, 337–350 (2007).

    Article  CAS  Google Scholar 

  10. G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, Elsevier (2013), 7th ed.

  11. A. Satoh, S. I. Imai, and L. Guarente, “The brain, sirtuins, and ageing,” Nat. Rev. Neurosci., 18, No. 6, 362–374 (2017), doi: https://doi.org/10.1038/nrn.2017.42.

    Article  CAS  PubMed  Google Scholar 

  12. P. Seoane-Collazo, J. Fernø, F. Gonzalez, et al., “Hypothalamicautonomic control of energy homeostasis,” Endocrine, 50, No. 2, 276–291 (2015), doi: https://doi.org/10.1007/s12020-015-0658-y.

    Article  CAS  PubMed  Google Scholar 

  13. B. Schwaller, “The regulation of a cell’s Ca(2+) signaling toolkit: the Ca (2+) homeostasome,” Adv. Exp. Med. Biol., 740, 1–25 (2012).

    Article  CAS  Google Scholar 

  14. Y. Zhang, M. S. Kim, B. Jia, et al., “Hypothalamic stem cells control ageing speed partly through exosomal miRNAs,” Nature, 548, No. 7665, 52–57 (2017), doi: https://doi.org/10.1038/nature23282.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Pathological Physiology, Yaroslavl State Medical University, Russian Ministry of Health, Yaroslavl, Russia

    V. V. Porseva

  2. Department of Normal Physiology, Yaroslavl State Medical University, Russian Ministry of Health, Yaroslavl, Russia

    M. B. Korzina, A. A. Spirichev, P. A. Vishnyakova, D. A. Aryaeva & P. M. Masliukov

  3. Department of General Physiology, St. Petersburg State University, St. Petersburg, Russia

    A. D. Nozdrachev

Authors
  1. V. V. Porseva
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  2. M. B. Korzina
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  3. A. A. Spirichev
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  4. P. A. Vishnyakova
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  5. D. A. Aryaeva
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  6. A. D. Nozdrachev
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  7. P. M. Masliukov
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Corresponding author

Correspondence to A. D. Nozdrachev.

Additional information

Translated from Morfologiya, Vol. 156, No. 5, pp. 39–43, September–October, 2019.

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Porseva, V.V., Korzina, M.B., Spirichev, A.A. et al. Changes in the Immunohistochemical Characteristics of Neurons in a Number of Hypothalamic Nuclei on Aging. Neurosci Behav Physi 50, 645–649 (2020). https://doi.org/10.1007/s11055-020-00947-6

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  • DOI: https://doi.org/10.1007/s11055-020-00947-6

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