Abstract
Seeds of Strychnos nux-vomica have slow and erratic germination; thus different presowing treatments were applied to enhance the germination of its seeds collected from Tamaraikulam, Tamil Nadu, India. In addition, the effects of desiccation and different storage conditions on the germination of S. nux-vomica seeds were investigated. The results show that soaking in 500 ppm gibberellic acid (GA3) for 24 h, incubation of seeds at 40 °C for 3 days and alternate water soaking (16 h) and drying (8 h) for 14 days significantly increased the percentage germination compared to the control. Desiccation of seeds down to 10% moisture content resulted in better germination. Germination of S. nux-vomica seeds differed significantly between different storage periods, moisture contents of the seed and for first and second order interactions (p<0.001). The highest germination (92%) was achieved when seeds with 10% moisture content were stored at ambient temperature for 30 weeks. Evidence from the present study indicates that S. nux-vomica seeds possess physiological dormancy that can be broken effectively by after-ripening. As seeds of S. nux-vomica are found to be desiccation tolerant, dry seed (10% moisture content) can be hermitically stored at ambient temperature for 30 weeks without losing their viability.
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References
American Cancer Society 2000. Guide to Complementary and Alternative Methods. American Cancer Society.
Asean Regional Centre for Biodiversity Conservation 2004. Asean's 100 Most Precious Plants. http://www.arcbc.org/arcbcweb/ASEAN_Precious_plants/poison/Strychnos nux-vomica.htm.
C.C. Baskin J.M. Baskin (1998) Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination Academic press San Diego 666
C.C. Baskin J.M. Baskin E.W. Chester (2001) ArticleTitleMorphological dormancy in seeds of Chamaelirium luteuma long-lived dioecious lily J. Torrey Bot. Soc. 128 7–15 Occurrence Handle10.2307/3088655
C.C. Baskin O. Zackrisson J.M. Baskin (2002) ArticleTitleRole of warm stratification in promoting germination of seeds of Empetrum hermaphroditum (Empetraceae), a circumboreal species with a stony endocarp Am. J. Bot. 89 486–493
J.D. Bewley M. Black (1994) Seeds: Physiology of Development and Germination Plenum press New York/London 445
Blanche C.A., Elam W.W., Hodges J.D., Bonner F.T. and Marquez A.C. 1988. Accelerated ageing of selected tree seeds. In: Werrall J., Loo-Dankins J. and Lester D.P. (eds.), Physiology and Genetics of Reforestation. Proc. of 10th North American Forest Biology Workshop, 20–22 July 1988, VancouverBC, pp. 327–334.
F.T. Bonner (1984) New forests from better seeds: the role of seed physiology M.L. Duryea G.N. Brown (Eds) Seedling Physiology and Reforestation Success Martinus Nijhoff/Dr. W Junk Publishers Dordrecht/Boston/Lancaster 37–59
K. Chaisurisri D.G.W. Edwards Y.A. El-Kassaby (1993) ArticleTitleAccelerated ageing of Sitka spruce seeds Silvae Genet. 42 303–308
Connor K.F. and Sowa S. 2002. Recalcitrant behavior of temperate forest tree seeds: storagebiochemistry and physiology. In: Outcalt K.W. (eds.), Proceedings of the eleventh biennial southern silvicultural research conference.
Danida Forest Seed Centre 1999. Desiccation and Storage Protocol. Newsletter No. 5: 23–40. Humlebaek, Denmark.
Danida Forest Seed Centre 2000. Strychnos Cocculoides. Newsletter No 7: 17–18. Humlebaek, Denmark.
A.J. Villiers ParticleDe M.W. Rooyen ParticleVan G.K. Theron (2002) ArticleTitleGermination strategies of Strandveld Succulent Karoo plant species for revegetation purposes: 1. Temperature and light requirements Seed Sci. & Technol. 30 17–33
Dey A.C. 1980. Indian Medicinal Plants Used in Ayurvedic Preparations. Bishen Singh and Mahendra Pal Singh Publishers.
B. Downie J.D. Bewley (1996) ArticleTitleDormancy in white spruce (Picea glauca (Moench.) Voss.) seeds are imposed by the tissue surrounding the embryo Seed Sci. Res. 6 9–15 Occurrence Handle10.1017/S0960258500002944
B. Downie H.W.M. Hillhorst J.D. Bewley (1997) ArticleTitleEndo-β-mannanase activity during dormancy alleviation and germination of white spruce (Picea glauca) seeds Physiol. Plantarum 101 405–415 Occurrence Handle1:CAS:528:DyaK2sXmvVWmur4%3D Occurrence Handle10.1111/j.1399-3054.1997.tb01015.x
J.P. Grime G. Mason A.V. Curtis J. Rodman S.R. Band M.A.G. Mowforth A.M. Neal S. Shaw (1981) ArticleTitleA comparative study of germination characteristics in a local flora J. Ecol. 69 1017–1059 Occurrence Handle10.2307/2259651
S.P.C. Groot C.M. Karssen (1987) ArticleTitleGibberellins regulate seed germination in tomato by endosperm weakening: A study with gibberellin-deficient mutants Planta 171 525–531 Occurrence Handle1:CAS:528:DyaL2sXlsFWgsbY%3D Occurrence Handle10.1007/BF00392302
S.N. Hidayati J.M. Baskin C.C. Baskin (2000a) ArticleTitleDormancy-breaking and germination requirements of seeds of four Lonicera species (Caprifoliaceae) with underdeveloped spatulate embryos Seed Sci. Res. 10 459–469
S.N. Hidayati J.M. Baskin C.C. Baskin (2000b) ArticleTitleDormancy-breaking and germination requirements for seeds of Diervilla lonicera (Caprifoliaceae), a species with underdeveloped linear embryos Can. J. Bot. 78 1199–1205 Occurrence Handle10.1139/cjb-78-9-1199
S.N. Hidayati J.M. Baskin C.C. Baskin (2002) ArticleTitleEffects of dry storage on germination and survivorship of seeds of Lonicera species (Caprifoliaceae) Seed Sci. & Technol. 30 137–148
InstitutionalAuthorNameInternational Seed Testing Association (2003) International Rules for Seed Testing International Seed Testing Association (ISTA) Bassersdorf, CH-Switzerland
C.M. Karssen (1995) Hormonal regulation of seed developmentdormancy and germination studied by genetic control J. Kigel G. Galili (Eds) Seed Development and Germination Marcel Dekker, Inc. New York/Basel/Hong Kong 333–350
T.N. Kaye (1997) Seed dormancy in high elevation plants: implications for ecology and restoration T.N. Kaye A. Liston R.M. Love D.L. Luoma R.J. Meinke M.V. Wilson (Eds) Conservation and Management of Native Plants and Fungi Native Plant Society of Oregon Corvallis, Oregon
Khadiwale V. 2000. An Estimate of Demand of Medicinal Plants by Herbal Industries and Ayurvedic Drug Producers of Maharashtra1991 Survey. http://planning commission.nic.in/aboutus/taskforce/tsk_medi.pdf.
T. Krishnamurthy (1993) Minor Forest Products of India Oxford and IBH Publ. Co. Pvt. Ltd Mumbai, India
C.L. Leadem (1987) ArticleTitleThe role of plant growth regulators in the germination of forest tree seeds Plant Growth Regul. 6 61–93 Occurrence Handle1:CAS:528:DyaL1cXnt1Sitg%3D%3D Occurrence Handle10.1007/BF00043950
P. Masilamani C. Dharmalingam (1998) ArticleTitleGermination improvement in teak (Tectona grandis Linn. f.) through forced ageing Curr. Sci. 75 356
S.S. Phartyal R.C. Thapliyal J.S. Nayal G. Joshi (2003a) ArticleTitleSeed storage physiology of Himalayan elm (Ulmus wallichiana): an endangered tree species of tropical highlands Seed Sci. & Technol. 31 651–658
S.S. Phartyal R.C. Thapliyal J.S. Nayal G. Joshi (2003b) ArticleTitleStorage of Himalayan maple (Acer caesium) seeds: A threatened tree species of the Central Himalayas Seed Sci. & Technol. 31 149–159
M.K. Sarcar A.B. Sarcar (1997) ArticleTitle Strychnos nux-vomica a medicinal plant Amruth 1 25
M. Schelin M. Tigabu I. Eriksson L. Sawadogo P.C. Odén (2003) ArticleTitleEffects of scarification, gibberellic acid and dry heat treatment on the germination of Balanites aegyptiaca seeds from Sudanian savanna in Burkina Faso Seed Sci. & Technol. 31 605–617
D. Teketay (1996) ArticleTitleGermination ecology of twelve indigenous and eight exotic multipurpose leguminous species from Ethiopia For. Ecol. Manage. 80 209–223 Occurrence Handle10.1016/0378-1127(95)03616-4
D.N. Tewari (2000) Report of the Task Force on Conservation & Sustainable use of Medicinal Plants Government of India Planning Commission March – 2000 India
M. Tigabu P.C. Odén (2001) ArticleTitleEffect of scarification, gibberellic acid and temperature on seed germination of two multipurpose Albizia species from Ethiopia Seed Sci. Technol. 29 11–20
C.W. Vertucci J.M. Farrant (1995) Acquisition and loss of desiccation tolerance J. Kigel G. Galili (Eds) Seed Development and Germination Marcel Dekker, Inc. New York/Basel/Hong Kong 237–271
T.V. Veselova V.A. Veselovsky (2003) ArticleTitleInvestigation of atypical changes during accelerated ageing of pea seeds Seed Sci. & Technol. 31 517–530
J.L. Walck C.C. Baskin J.M. Baskin (1999) ArticleTitleSeeds of Thalictrum mirabile (Ranunculaceae) require cold stratification for loss of non-deep simple morphological dormancy Can. J. Bot. 77 1769–1776 Occurrence Handle10.1139/cjb-77-12-1769
J. Zar (1996) Biostatistical Analysis Prentice - Hall Inc New Jersey 662
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Sivakumar, V., Anandalakshmi, R., Warrier, R.R. et al. Effects of presowing treatments, desiccation and storage conditions on germination of Strychnos nux-vomica seeds, a valuable medicinal plant. New Forest 32, 121–131 (2006). https://doi.org/10.1007/s11056-005-5038-7
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DOI: https://doi.org/10.1007/s11056-005-5038-7